控制抗菊粉抗体反应多样性的Sr1基因定位于小鼠第14号染色体。

The Sr1 gene that controls diversity of the anti-inulin antibody response maps to mouse chromosome 14.

作者信息

Jones Tiffany Linda, Riblet Roy, Stein Kathryn E

机构信息

Division of Monoclonal Antibodies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.

出版信息

Immunogenetics. 2003 May;55(2):80-6. doi: 10.1007/s00251-003-0555-z. Epub 2003 Apr 9.

DOI:10.1007/s00251-003-0555-z

PMID:12684850

Abstract

Previous studies demonstrated that the diversity of the antibody response of mice to the inulin (In) determinant of bacterial levan is regulated by the gene Spectrotype Regulation 1 ( Sr1). BALB/c mice produce a monoclonal anti-In response as shown by isoelectric focusing analysis. In contrast, the anti-In antibody response of (BALB/cxC57BL/6)F1 mice is significantly more heterogeneous. We performed a backcross and a genome-wide scan with microsatellite markers and found that Sr1 is tightly linked to D14Mit121 on chromosome (Chr) 14. This location for Sr1 was supported by analysis of CXB Recombinant Inbred strains. We further confirmed this by finding that the Chr 14 congenic mouse strain B6.C-H8 lacks the C57BL/6 allele of the Sr1 gene, indicating that Sr1 is located in the segment of Chr 14 replaced with BALB/c donor DNA. These data place Sr1 near to or coincident with the Tcra/Tcrd T-cell receptor gene complex and suggest a role for T cells in diversifying the anti-In response.

摘要

先前的研究表明，小鼠对细菌果聚糖的菊粉（In）决定簇的抗体反应多样性受基因光谱型调节1（Sr1）调控。如等电聚焦分析所示，BALB/c小鼠产生单克隆抗In反应。相比之下，（BALB/cxC57BL/6）F1小鼠的抗In抗体反应明显更具异质性。我们进行了回交并用微卫星标记进行全基因组扫描，发现Sr1与14号染色体（Chr）上的D14Mit121紧密连锁。CXB重组近交系的分析支持了Sr1的这一位置。我们通过发现14号染色体同源基因小鼠品系B6.C-H8缺乏Sr1基因的C57BL/6等位基因进一步证实了这一点，这表明Sr1位于14号染色体被BALB/c供体DNA取代的片段中。这些数据表明Sr1靠近或与Tcra/Tcrd T细胞受体基因复合体重合，并提示T细胞在使抗In反应多样化中发挥作用。