Lieberman R, Potter M, Humphrey W, Chien C C
J Immunol. 1976 Dec;117(6):2105-11.
The serum from non-immunized mice of strains BALB/c, C58, A/He, and RIII contained hemagglutinins for stearoyl inulin-coated SRBC. Immunization with bacterial levan slightly elevated these titers. These same sera also carried cross-specific idiotypic determinants (IdX) that are associated only with inulin-binding myeloma proteins (INBMP) of BLAB/c mice. Three InuldX specificities, A, B, and G, were identified. The InuIdX phenotypes of strains BALB/c, C58, and A/He were InuIdXA+B+G+; strain RIII was InuIdX A+B-G+; strain C57BL/6, C57BL/10, DBA/2, AKR and NH were A-B-G-. Strains CBA, C3H, PL, and C57L could not be typed because of low and inconsistent levels of InuIdX and anti-inulin hemagglutinins. The InuIdXA+B+G+ phenotype was used as a genetic marker in immunoglobulin congenic strains CB-20, BAB-14, and BC-8 and in Bailey RI strains which are derived from crosses of BALB/c (InuIdXA+B+G+) and C57BL/ka or C57BL/6, respectively (InuldXA-B-G-). Linkage of the IdXA+B+G+ to the BALB/c a allotype locus was demonstrated. In addition, the InuldXA+B+G+ marker was used as a phenotype in an analysis of 168 first generation backcorss progeny (C57BL X (C57BL X BALB/c) F). Linkage of the marker to the BALB/c allotype was found again. Two proven recombinant mice having the C57BL a allotype and the InuIdxA+B+G+ markers were identified and progeny tested. Four other potential crossover types are still being progeny-tested.
来自BALB/c、C58、A/He和RIII品系未免疫小鼠的血清含有针对硬脂酰菊粉包被的绵羊红细胞（SRBC）的血凝素。用细菌果聚糖免疫可使这些滴度略有升高。这些相同的血清还带有交叉特异性独特型决定簇（IdX），这些决定簇仅与BALB/c小鼠的菊粉结合骨髓瘤蛋白（INBMP）相关。鉴定出三种InuIdX特异性，即A、B和G。BALB/c、C58和A/He品系的InuIdX表型为InuIdXA+B+G+；RIII品系为InuIdX A+B-G+；C57BL/6、C57BL/10、DBA/2、AKR和NH品系为A-B-G-。CBA、C3H、PL和C57L品系由于InuIdX和抗菊粉血凝素水平低且不一致而无法分型。InuIdXA+B+G+表型被用作免疫球蛋白同基因品系CB-20、BAB-14和BC-8以及贝利RI品系的遗传标记，贝利RI品系分别来自BALB/c（InuIdXA+B+G+）与C57BL/ka或C57BL/6（InuldXA-B-G-）的杂交。证明了IdXA+B+G+与BALB/c a同种异型位点的连锁关系。此外，InuldXA+B+G+标记在对168个第一代回交后代（C57BL X（C57BL X BALB/c）F）的分析中用作表型。再次发现该标记与BALB/c同种异型的连锁关系。鉴定出两只具有C57BL a同种异型和InuIdxA+B+G+标记的经证实的重组小鼠并进行了子代测试。其他四种潜在的交叉类型仍在进行子代测试。
