Bork Sabine, Yokoyama Naoaki, Matsuo Tomohide, Claveria Florencia G, Fujisaki Kozo, Igarashi Ikuo
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
Am J Trop Med Hyg. 2003 Mar;68(3):334-40.
We evaluated the growth inhibitory effect of triclosan, which has recently been reported to inhibit the growth of Plasmodium species and Toxoplasma gondii, on bovine and equine Babesia parasites in in vitro cultures The growth of Babesia bovis and B. bigemina was significantly inhibited in the presence of 100 microg/ml of triclosan, while B. caballi and B. equi were susceptible to as low as 50 microg/ml. Babesia bigemina and B. caballi were completely cleared as early as on the first and second day of the treatment, respectively. These parasites did not exhibit any growth in the subsequent five-day period of subculture without triclosan. Drug-treated parasites appeared pycnotic and atypically shaped, and ultrastructurally showed pronounced vacuolations, leading to complete destruction of parasites. Light microscopy showed that used concentrations of triclosan showed no toxicity against the host cells. The results suggest that triclosan can be used for chemotherapy of babesiosis.
我们评估了三氯生对牛和马巴贝斯虫寄生虫在体外培养中的生长抑制作用，最近有报道称三氯生可抑制疟原虫和弓形虫的生长。在100微克/毫升的三氯生存在下，牛巴贝斯虫和双芽巴贝斯虫的生长受到显著抑制，而马巴贝斯虫和驽巴贝斯虫对低至50微克/毫升的三氯生敏感。双芽巴贝斯虫和马巴贝斯虫分别在治疗的第一天和第二天就被完全清除。在随后五天无三氯生的传代培养期间，这些寄生虫没有出现任何生长。经药物处理的寄生虫呈现核固缩且形状异常，超微结构显示有明显的空泡化，导致寄生虫完全被破坏。光学显微镜检查表明，所用浓度的三氯生对宿主细胞没有毒性。结果表明，三氯生可用于巴贝斯虫病的化疗。
