Saidon Jacob, He Jianing, Zhu Qiang, Safavi Kamran, Spångberg Larz S W
University of Connecticut Health Center, School of Dental Medicine, Department of Endodontology, 263 Farmington Ave MC-1715, Farmington, CT 06030, USA.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003 Apr;95(4):483-9. doi: 10.1067/moe.2003.20.
Mineral trioxide aggregate (MTA) is being widely used for root-end fillings, pulp capping, perforation repairs, and other endodontic procedures. MTA and Portland cement (PC) have many similar physical, chemical, and biologic properties. PC cement has shown promising potential as an endodontic material in several studies in vitro and in vivo. The purpose of this study was to compare the cytotoxic effect in vitro and the tissue reaction of MTA and Portland cement in bone implantation in the mandibles of guinea pigs.
Millipore culture plate inserts with freshly mixed or set material were placed into the culture plates with already attached L929 cells. After an incubation period of 3 days, the cell morphology and cell counts were studied. Adult male guinea pigs under strict asepsis were anesthetized, during which a submandibular incision was made to expose the symphysis of the mandible. Bilateral bone cavities were prepared and Teflon applicators with freshly mixed materials were inserted into the bone cavities. Each animal received 2 implants, one filled with ProRoot and 1 with PC. The animals were killed after 2 or 12 weeks, and the tissues were processed for histologic evaluation by means of light microscopy.
There was no difference in cell reactions in vitro. Bone healing and minimal inflammatory response adjacent to ProRoot and PC implants were observed in both experimental periods, suggesting that both materials are well tolerated.
MTA and PC show comparative biocompatibility when evaluated in vitro and in vivo. The results suggest that PC has the potential to be used as a less expensive root-end filling material.
矿物三氧化物凝聚体（MTA）被广泛用于根尖倒充填、牙髓盖髓、穿孔修补及其他牙髓治疗程序。MTA与波特兰水泥（PC）具有许多相似的物理、化学和生物学特性。在多项体外和体内研究中，PC水泥已显示出作为牙髓材料的良好潜力。本研究的目的是比较MTA和PC水泥在豚鼠下颌骨植入时的体外细胞毒性作用及组织反应。
将装有新鲜混合或凝固材料的密理博培养板插入物放入已接种L929细胞的培养板中。孵育3天后，研究细胞形态和细胞计数。对严格无菌条件下的成年雄性豚鼠进行麻醉，期间做下颌下切口以暴露下颌骨联合处。制备双侧骨腔，并将装有新鲜混合材料的聚四氟乙烯涂抹器插入骨腔。每只动物植入2个材料，一个填充ProRoot，另一个填充PC。在2周或12周后处死动物，对组织进行处理以通过光学显微镜进行组织学评估。
体外细胞反应无差异。在两个实验阶段均观察到ProRoot和PC植入物附近的骨愈合及最小炎症反应，表明两种材料均具有良好的耐受性。
在体外和体内评估时，MTA和PC显示出相当的生物相容性。结果表明PC有潜力用作较便宜的根尖倒充填材料。
