Gomes-Filho João Eduardo, Rodrigues Guilherme, Watanabe Simone, Estrada Bernabé Pedro Felício, Lodi Carolina Simonett, Gomes Alessandra Cristina, Faria Max Doulgas, Domingos Dos Santos Alailson, Silos Moraes João Carlos
Department of Endodontics, Araçatuba School of Dentistry, São Paulo State University, Araçatuba, São Paulo, Brazil.
J Endod. 2009 Oct;35(10):1377-80. doi: 10.1016/j.joen.2009.06.010. Epub 2009 Aug 6.
A new cement (CER; Cimento Endodôntico Rápido or fast endodontic cement) has been developed to improve handling properties. It is a formulation that has Portland cement in gel. However, there had not yet been any study evaluating its biologic properties. The purpose of this study was to evaluate the rat subcutaneous tissue response to CER and Angelus MTA.
The materials were placed in polyethylene tubes and implanted into dorsal connective tissue of Wistar rats for 7, 30, and 60 days. The specimens were prepared to be stained with hematoxylin-eosin or von Kossa or not stained for polarized light. The presence of inflammation, predominant cell type, calcification, and thickness of fibrous connective tissue were recorded. Scores were defined as follows: 0, none or few inflammatory cells, no reaction; 1, <25 cells, mild reaction; 2, 25-125 cells, moderate reaction; 3, >125 cells, severe reaction. Fibrous capsule was categorized as thin when thickness was <150 mum and thick at >150 mum. Necrosis and formation of calcification were both recorded.
Both materials Angelus MTA and CER caused moderate reactions at 7 days, which decreased with time. The response was similar to the control at 30 and 60 days with Angelus MTA and CER, characterized by organized connective tissue and presence of some chronic inflammatory cells. Mineralization and granulations birefringent to polarized light were observed with both materials.
It was possible to conclude that CER was biocompatible and stimulated mineralization.
已研发出一种新型水门汀（CER；快速牙髓水门汀或牙髓快速水门汀）以改善操作性能。它是一种含有凝胶状波特兰水泥的配方。然而，尚未有任何研究评估其生物学特性。本研究的目的是评估大鼠皮下组织对CER和安捷罗斯MTA的反应。
将材料置于聚乙烯管中，植入Wistar大鼠的背部结缔组织7天、30天和60天。制备标本以进行苏木精 - 伊红染色或冯·科萨染色，或不染色用于偏振光观察。记录炎症的存在、主要细胞类型、钙化情况以及纤维结缔组织的厚度。评分定义如下：0分，无或极少炎性细胞，无反应；1分，<25个细胞，轻度反应；2分，25 - 125个细胞，中度反应；3分，>125个细胞，重度反应。当纤维囊厚度<150μm时分类为薄，>150μm时分类为厚。记录坏死和钙化形成情况。
安捷罗斯MTA和CER这两种材料在7天时均引起中度反应，随时间推移反应减弱。在30天和60天时，安捷罗斯MTA和CER的反应与对照组相似，其特征为有组织的结缔组织和一些慢性炎性细胞的存在。两种材料均观察到矿化和对偏振光双折射的颗粒。
可以得出结论，CER具有生物相容性并能刺激矿化。
