Chailakhyan Ruben K., Guerasimov Yuriy V., Kuralesova Albina I., Latzinik Natalia V., Genkina Elena N.
Gamaleya Institute for Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, Russia.
Russ J Immunol. 2000 Jul;5(2):141-148.
When heterotopically transplanted under the renal capsule, the strains of stromal fibroblasts from spleen cultures formed the lymphoid organs, which had no specialized structures, such as red and white pulp and consisted of the accumulations like lymphoid follicles. The purpose of the given work was the study of ability of spleen stromal fibroblast strains, when heterotopically transplanted into the organism, to transfer the specific lymphoid microenvironment. The heterotopic transplantation of spleen fragments and stromal cells, grown in vitro, was made in autologous variant. The spleen fragments were put under a renal capsule. The strains of stromal fibroblasts of 5-7th passages were put into porous frames under a renal capsule of normal or immunized rabbits. The immune response to sheep red blood cells was studied in spleen of normal and hyperimmune rabbits, in regenerating spleens, in heterotopic grafts of spleen and in lymphoid organs, formed by strains of spleen stromal fibroblasts, grown in cultures. The immune response was determined on the 7-8th day after immunization by counting the amount of plaque forming cells (PFC) by Jerne method. In lymphoid organs, formed at the place of transplantation of spleen stromal fibroblasts strains of rabbits, the number of PFC was almost 3 times lower, than in heterotopic transplants of spleen, and 6 times lower, than in normal and regenerating spleens, but hundreds times exceeding the number of spontaneous PFC which could be only found, when 10(7)-10(8) spleen cells of normal non-immunized animals were tested. The concentration of PFC in lymphoid organs, formed at the place of the graft of stromal fibroblasts, was 3 times lower in regenerating spleens of hyperimmunised animals and 5 times higher, than in transplants of stromal fibroblasts of normal spleen. The obtained results give an evidence, that the transplantation of spleen stromal fibroblasts provides the transfer of specific lymphoid microenvironment.
当在肾被膜下进行异位移植时，来自脾培养物的基质成纤维细胞株形成了淋巴器官，这些淋巴器官没有诸如红髓和白髓等特殊结构，而是由类似淋巴滤泡的聚集物组成。这项工作的目的是研究脾基质成纤维细胞株在异位移植到生物体内时转移特定淋巴微环境的能力。脾碎片和体外培养的基质细胞的异位移植采用自体移植方式。将脾碎片置于肾被膜下。将第5 - 7代的基质成纤维细胞株置于正常或免疫兔子的肾被膜下的多孔框架中。在正常和超免疫兔子的脾脏、再生脾、脾的异位移植物以及由培养的脾基质成纤维细胞株形成的淋巴器官中研究对绵羊红细胞的免疫反应。在免疫后第7 - 8天，通过Jerne方法计数噬斑形成细胞（PFC）的数量来确定免疫反应。在兔子脾基质成纤维细胞株移植部位形成的淋巴器官中，PFC的数量比脾的异位移植中几乎低3倍，比正常和再生脾中低6倍，但比仅在检测10(7)-10(8)个正常未免疫动物的脾细胞时能发现的自发PFC数量高出数百倍。在超免疫动物再生脾中，在基质成纤维细胞移植部位形成的淋巴器官中PFC的浓度比正常脾基质成纤维细胞移植物低3倍，比其高5倍。所获得的结果证明，脾基质成纤维细胞的移植能实现特定淋巴微环境的转移。
