Sedgley M, Ford W L
Cell Tissue Kinet. 1976 May;9(3):231-43. doi: 10.1111/j.1365-2184.1976.tb01271.x.
The chain of lymph-nodes in the rat mesentery was isolated and the preparation was perfused via cannulae in the superior mesenteric vessels. The perfusate consisted of serum to which labelled lymphocytes had usually been added. The entry of radioactively labelled lymphocytes from the blood vessels into the lymph-nodes was studied by scintillation counting and autoradiography. It was observed that: (1) In the perfused node labelled lymphocytes localized in and around post-capillary venules in the paracortex as they do early after i.v. injection. (2) The number of lymphocytes which entered the node was directly proportional to the concentration in the perfusate over the range studied. The proportion of cells retained in the node varied considerably around a mean of 11% of lymphocytes reaching it. (3) The isolated lymph-node released few if any lymphocytes into the effluent (venous) perfusate. (4) Large lymphocytes migrated into isolated lymph-nodes slightly more readliy than did small lymphocytes. (5) Unlike intact cells isolated lymphocyte membranes did not adhere to specialized vascular endothelium.
分离大鼠肠系膜中的淋巴结链，并通过肠系膜上血管中的插管对标本进行灌注。灌注液为通常添加了标记淋巴细胞的血清。通过闪烁计数和放射自显影研究放射性标记淋巴细胞从血管进入淋巴结的情况。观察到：（1）在灌注的淋巴结中，标记淋巴细胞如同静脉注射后早期那样定位于副皮质区毛细血管后微静脉及其周围。（2）在所研究的浓度范围内，进入淋巴结的淋巴细胞数量与灌注液中的浓度成正比。到达淋巴结的淋巴细胞中，保留在淋巴结中的细胞比例在平均值11%左右有很大差异。（3）分离的淋巴结向流出（静脉）灌注液中释放的淋巴细胞极少，甚至没有。（4）大淋巴细胞比小淋巴细胞稍微更容易迁移到分离的淋巴结中。（5）与完整细胞不同，分离的淋巴细胞膜不黏附于特化的血管内皮。
