乙型肝炎病毒包膜蛋白的N糖基化在丁型肝炎病毒形态发生和感染性中的作用
Role of N glycosylation of hepatitis B virus envelope proteins in morphogenesis and infectivity of hepatitis delta virus.
作者信息
Sureau Camille, Fournier-Wirth Chantal, Maurel Patrick
机构信息
Laboratoire de Virologie Moléculaire, INSERM U76, INTS, Paris, France.
出版信息
J Virol. 2003 May;77(9):5519-23. doi: 10.1128/jvi.77.9.5519-5523.2003.
Abstract
Hepatitis delta virus (HDV) particles are coated with the large (L), middle (M), and small (S) hepatitis B virus envelope proteins. In the present study, we constructed glycosylation-defective envelope protein mutants and evaluated their capacity to assist in the maturation of infectious HDV in vitro. We observed that the removal of N-linked carbohydrates on the S, M, and L proteins was tolerated for the assembly of subviral hepatitis B virus (HBV) particles but was partially inhibitory for the formation of HDV virions. However, when assayed on primary cultures of human hepatocytes, virions coated with S, M, and L proteins lacking N-linked glycans were infectious. Furthermore, in the absence of M, HDV particles coated with nonglycosylated S and L proteins retained infectivity. These results indicate that carbohydrates on the HBV envelope proteins are not essential for the in vitro infectivity of HDV.
摘要
丁型肝炎病毒（HDV）颗粒被乙肝病毒的大（L）、中（M）、小（S）包膜蛋白包裹。在本研究中，我们构建了糖基化缺陷的包膜蛋白突变体，并评估了它们在体外协助感染性HDV成熟的能力。我们观察到，S、M和L蛋白上N-连接碳水化合物的去除对于亚病毒乙肝病毒（HBV）颗粒的组装是可以耐受的，但对HDV病毒粒子的形成有部分抑制作用。然而，当在原代人肝细胞培养物上进行检测时，包裹着缺乏N-连接聚糖的S、M和L蛋白的病毒粒子具有感染性。此外，在没有M蛋白的情况下，包裹着非糖基化S和L蛋白的HDV颗粒仍具有感染性。这些结果表明，乙肝病毒包膜蛋白上的碳水化合物对于HDV的体外感染性并非必不可少。
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