Komatsu Suguru, Sasaki Yasuo, Shiota Kohei
Congenital Anomaly Research Center, Kyoto University Graduate School of Medicine, Yoshida Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.
Congenit Anom (Kyoto). 2003 Mar;43(1):41-5. doi: 10.1111/j.1741-4520.2003.tb01025.x.
Pregnant ICR mice were given 20% ethanol intraperitoneally twice on day 13 of gestation and allowed to give birth to offspring. The offspring were killed at 56 days of age and the motor root of their facial nerve was examined histologically and morphometrically. The cross-sectional area of the facial nerve of mice prenatally exposed to ethanol was significantly smaller than that of the control mice. There was no significant difference in the total number of myelinated axons or the mean axonal diameter between control and ethanol-exposed mice, but the mean diameter of myelinated fibers (axon + myelin sheath) and the thickness of myelin sheath were significantly decreased in the treated group. These results suggest that prenatal exposure to ethanol disturbs myelination of the motor root of the facial nerve and may cause permanent neurological effects.
妊娠第13天，对怀孕的ICR小鼠腹腔注射20%乙醇，共两次，然后让其产仔。子代小鼠在56日龄时处死，对其面神经运动根进行组织学和形态计量学检查。产前暴露于乙醇的小鼠面神经横截面积显著小于对照小鼠。对照小鼠和暴露于乙醇的小鼠之间，有髓轴突总数或平均轴突直径无显著差异，但治疗组有髓纤维（轴突+髓鞘）的平均直径和髓鞘厚度显著降低。这些结果表明，产前暴露于乙醇会干扰面神经运动根的髓鞘形成，并可能导致永久性神经学影响。
