无血清培养基中犬间充质基质细胞衍生的细胞外囊泡对小胶质细胞具有抗炎作用。
Extracellular Vesicles Derived From Canine Mesenchymal Stromal Cells in Serum Free Culture Medium Have Anti-inflammatory Effect on Microglial Cells.
作者信息
Kuwahara Yukina, Yoshizaki Karin, Nishida Hidetaka, Kamishina Hiroaki, Maeda Sadatoshi, Takano Katsura, Fujita Naoki, Nishimura Ryohei, Jo Jun-Ichiro, Tabata Yasuhiko, Akiyoshi Hideo
机构信息
Joint Department of Veterinary Medicine, The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.
Department of Veterinary Surgery, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Japan.
出版信息
Front Vet Sci. 2021 Apr 28;8:633426. doi: 10.3389/fvets.2021.633426. eCollection 2021.
Mesenchymal stem/stromal cells (MSCs) have been used as cell sources for treating dogs with naturally-occurring diseases. Extracellular vesicles (EVs) derived from MSCs are now recognized as pivotal to modulating the immune response and supporting tissue repair. Manufacture of MSC-EVs for clinical application mandates removal of the xeno-proteins, including fetal bovine serum. The objective of this study was to examine whether canine MSCs survived and secreted EVs in serum-free medium (SFM) conditions and to assess the immunomodulatory effect of EVs . Canine MSCs were found to survive and secrete EVs under SFM conditions. The surface markers of MSCs in the SFM were similar to MSCs in complete culture medium. Canine MSC-EVs had a diameter of ~300 nm and were positive for EV markers. MSC-derived EVs from the serum-free condition reduced the levels of β by BV-2 cells in response to LPS stimulation. These results warrant further studies of the use of SFM for producing EVs derived from canine MSCs.
间充质干/基质细胞（MSCs）已被用作治疗患有自然发生疾病的犬类的细胞来源。源自MSCs的细胞外囊泡（EVs）现在被认为对调节免疫反应和支持组织修复至关重要。用于临床应用的MSC-EVs的制造要求去除异种蛋白，包括胎牛血清。本研究的目的是检查犬MSCs在无血清培养基（SFM）条件下是否存活并分泌EVs，并评估EVs的免疫调节作用。发现犬MSCs在SFM条件下存活并分泌EVs。SFM中MSCs的表面标志物与完全培养基中的MSCs相似。犬MSC-EVs的直径约为300nm，且EV标志物呈阳性。无血清条件下源自MSCs的EVs降低了BV-2细胞对LPS刺激的β水平。这些结果值得进一步研究使用SFM生产源自犬MSCs的EVs。
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