[玉米自交系P25抗南方锈病基因的遗传分析及SSR连锁标记定位研究]

[Studies of genetic analysis and SSR linked marker location of gene resistance to southern rust in inbred line P25 of maize].

作者信息

Liu Zhang-Xiong, Wang Shou-Cai, Dai Jing-Rui, Huang Lie-Jian, Cao Hai-He

机构信息

Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100094, China.

出版信息

Yi Chuan Xue Bao. 2003 Aug;30(8):706-10.

PMID:14682237

Abstract

Using P25 (immune inbred line), F349 (susceptible inbred line) and the derived population F1, F2, B1 and B2 as materials, we investigated the heredity the of disease resistance gene to maize southern rust (Puccinia polysora Underw.) through the major-gene and polygene inheritance model. The results indicate that a major resistance gene exists in the inbred line P25 and expresses with additive effect. We didn't find any multi-genes. The inheritabilities of this major resistant gene among F2, B1 and B2 were 81.88%, 38.14% and 55.1%, respectively. We constructed a maize SSR linkage map using P25 x F349 F2:3 population, and located the resistant gene on chromosome 10. The genetic distance between this gene and phi059 marker was 5.8 cM.

摘要

以P25（免疫自交系）、F349（感病自交系）及其衍生群体F1、F2、B1和B2为材料，通过主基因-多基因遗传模型研究了玉米抗南方锈病基因（Puccinia polysora Underw.）的遗传规律。结果表明，自交系P25中存在一个主效抗病基因，表现为加性效应，未发现多基因。该主效抗病基因在F2、B1和B2中的遗传率分别为81.88%、38.14%和55.1%。利用P25×F349 F2:3群体构建了玉米SSR连锁图谱，并将抗病基因定位在第10号染色体上，该基因与phi059标记的遗传距离为5.8 cM。