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摇蚊多线染色体带的DNA含量加倍后复制时间的延长。

Prolongation of replication time after doublings of the DNA content of polytene chromosome bands of Chironomus.

作者信息

Hägele K

出版信息

Chromosoma. 1976 May 12;55(3):253-8. doi: 10.1007/BF00300391.

DOI:10.1007/BF00300391
PMID:1269343
Abstract

Using 3H-thymidine autoradiography, labeling frequency of homologous asynapsed chromosome bands of the hybrid of Chironomus th. thummi and Chironomus th. piger has been studied. In a number of these bands the DNA content of the thummi bands if 2, 4, 8 or 16 times as large as that of the homologous piger bands (Keyl, 1965). Those bands of CH. TH. thummi which show one doubling of their DNA content in comparison with the homologous piger bands are also labeled two times more frequently than piger. In contrast to this such a correlation between increase of labeling frequency (i.e. prolongation of replication time) and doubling of the DNA content is not observed, when thummi bands have 4, 8 or 16 times more DNA than their homologues in piger. In these cases replication time is also prolonged after each doubling. Duration of DNA synthesis increases linearly but always by a smaller factor as the corresponding DNA content is increased.

摘要

利用³H-胸腺嘧啶放射自显影技术,对摇蚊(Chironomus th. thummi)与摇蚊(Chironomus th. piger)杂交种同源未联会染色体带的标记频率进行了研究。在许多这样的带中,thummi带的DNA含量是同源piger带的2、4、8或16倍(凯尔,1965)。与同源piger带相比,CH. TH. thummi中那些DNA含量加倍的带,其标记频率也比piger带高两倍。与此相反,当thummi带的DNA比其在piger中的同源带多4、8或16倍时,未观察到标记频率增加(即复制时间延长)与DNA含量加倍之间的这种相关性。在这些情况下,每次加倍后复制时间也会延长。DNA合成持续时间呈线性增加,但随着相应DNA含量的增加,增加的系数总是较小。

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引用本文的文献

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Double-stranded DNA with the size expected of replicons can be released from Chironomus polytene chromosomes.具有复制子预期大小的双链DNA可从摇蚊多线染色体中释放出来。
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3
Differential staining of polytene chromosome bands in Chironomus by Giemsa banding methods.用吉姆萨显带法对摇蚊多线染色体带进行差异染色。

本文引用的文献

1
Karyotypes of Chironomus thummi. I. Diagram of the salivary chromosomes of Chironomus thummi thummi and cytological differentiation of the subspecies Chiromomus thummi thummi and Chironomus thummi piger.图姆氏摇蚊的核型。I. 图姆氏摇蚊指名亚种的唾液腺染色体图谱以及图姆氏摇蚊指名亚种和图姆氏摇蚊猪亚种的细胞学分化
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Chromosoma. 1977 Feb 3;59(3):207-16. doi: 10.1007/BF00292778.
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Chromosoma. 1972;39(1):63-82. doi: 10.1007/BF00320591.
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