Hägele K
Chromosoma. 1977 Feb 3;59(3):207-16. doi: 10.1007/BF00292778.
Two Giemsa banding methods (C banding and RB banding) are described which selectively stain the centromere bands of polytene salivary gland chromosomes in a number of Chironomus species. - By the C banding method the polytene chromosome appearance is changed grossly. Chromosome bands, as far as they are identifiable, are stained pale with the exception of the centromere bands and in some cases telomeres, which then are intensely stained reddish blue. - By the RB method the centromere bands are stained bright blue, whereas the remainder of the polytene bands stain red to red-violet. - Contrary to all other species examined, in Chironomus th. thummi numerous interstitial polytene chromosome bands, in addition to the centromere regions, are positively C banded and blue stained by RB banding. In the hybrid of Ch. th. thummi X Ch. th. piger only those interstitial thummi bands which are known to have a greater DNA content than their homologous piger bands are C banding positive and blue stained by the RB method whereas the homologous piger bands are C banding negative and red stained by RB banding. Ch. thummi and piger bands with an equal amount of DNA both show no C banding and stain red by RB banding. - It seems that the Giemsa banding methods used are capable of demonstrating, in addition to centromeric heterochromatin, heterochromatin in those interstitial polytene chromosome bands whose DNA content has been increased during chromosome evolution.
本文描述了两种吉姆萨显带方法（C带和RB带），它们可选择性地对多种摇蚊唾液腺多线染色体的着丝粒带进行染色。——通过C带方法，多线染色体的外观发生了显著变化。除着丝粒带以及在某些情况下的端粒带被强烈染成红蓝色外，染色体带（只要能识别）均被淡染。——通过RB方法，着丝粒带被染成亮蓝色，而多线染色体带的其余部分则染成红色至红紫色。——与所有其他已检测的物种不同，在嗜尸摇蚊中，除着丝粒区域外，许多间质多线染色体带呈C带阳性，并通过RB带染成蓝色。在嗜尸摇蚊×猪型摇蚊的杂交种中，只有那些已知DNA含量高于其同源猪型摇蚊带的间质嗜尸摇蚊带呈C带阳性，并通过RB方法染成蓝色，而同源猪型摇蚊带则呈C带阴性，并通过RB带染成红色。DNA含量相等的嗜尸摇蚊带和猪型摇蚊带均未显示C带，且通过RB带染成红色。——似乎所使用的吉姆萨显带方法除了能显示着丝粒异染色质外，还能显示那些在染色体进化过程中DNA含量增加的间质多线染色体带中的异染色质。
