Inglis Neil F, Stevenson Karen, Heaslip Darragh G, Sharp J Michael
Division of Bacteriology, Moredun Research Institute, International Research Centre, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK.
FEMS Microbiol Lett. 2003 Apr 11;221(1):39-47. doi: 10.1016/S0378-1097(03)00136-8.
Data are presented on the identification and characterisation of 17 chromosomal integration loci of the insertion element IS901 in the Mycobacterium avium (cervine strain JD88/118) genome. Thirteen of these integration loci have been mapped to their corresponding positions on the M. avium strain 104 (an IS901(-) strain) genome (The Institute for Genome Research (TIGR) unfinished genome-sequencing project). Sequence data for both upstream and downstream sequence flanking regions were obtained for 12 insertion loci, while upstream sequence was obtained for five others. A consensus IS901 insertion target sequence compiled from all 17 integration sites was in broad agreement with earlier reports that were based on only two such loci. Analysis of IS901 integration site flanking sequences revealed that, like IS900 in M. avium subspecies paratuberculosis, IS901 inserts preferentially between a putative ribosome-binding sequence (RBS) and the translational start codon of an open reading frame (ORF). In BLAST X and BLAST P searches of the GenBank database, these ORFs were shown to share significant homologies with a number of other prokaryotic genes.
本文展示了关于插入元件IS901在鸟分枝杆菌（鹿种菌株JD88/118）基因组中的17个染色体整合位点的鉴定和特征分析数据。其中13个整合位点已被定位到鸟分枝杆菌菌株104（一株IS901阴性菌株）基因组上的相应位置（美国基因组研究所（TIGR）未完成的基因组测序项目）。获得了12个插入位点两侧上游和下游序列的序列数据，另外5个只获得了上游序列。从所有17个整合位点汇编得到的IS901插入靶序列共识与早期仅基于两个此类位点的报道基本一致。对IS901整合位点侧翼序列的分析表明，与副结核分枝杆菌亚种中的IS900一样，IS901优先插入假定的核糖体结合序列（RBS）与开放阅读框（ORF）的翻译起始密码子之间。在对GenBank数据库进行的BLAST X和BLAST P搜索中，这些ORF与许多其他原核基因具有显著的同源性。
