Gunawardena N K, Fujimaki Y, Aoki Y
Department of Parasitology, Institute of Tropical Medicine, Nagasaki University, Sakamoto, 852-8523 Nagasaki, Japan.
Parasitol Res. 2003 Jul;90(4):337-42. doi: 10.1007/s00436-003-0838-1. Epub 2003 Apr 15.
The Brugia pahangi infective larval response to jird serum was studied using an agar plate assay. Larvae placed onto the agar remained at the same place for 60 min. Once the larvae were stimulated by serum, more than 95% oriented towards the serum and reached it within few minutes. This larval response was inhibited by an activator of phosphodiesterase (imidazole), adenylate cyclase inhibitors (SQ22536 and MDL-12330A) and protein kinase A inhibitor. An inhibitor of phosphodiesterase (IBMX), an activator of adenylate cyclase (forskolin) and an membrane permeant analogue of cAMP (8-bromo-cAMP), caused a number of larvae to move out from the inoculation area towards the other zones. To our knowledge, this is the first report of a chemotactic response by B. pahangi larvae to host serum. We conclude that B. pahangi larvae show a chemotaxic response to host serum, and that cAMP and cAMP dependent protein kinase are involved in the signal transduction.
使用琼脂平板试验研究了彭亨布鲁线虫感染性幼虫对沙鼠血清的反应。置于琼脂上的幼虫在同一位置停留60分钟。一旦幼虫受到血清刺激，超过95%的幼虫会朝着血清方向移动，并在几分钟内到达血清处。这种幼虫反应受到磷酸二酯酶激活剂（咪唑）、腺苷酸环化酶抑制剂（SQ22536和MDL-12330A）和蛋白激酶A抑制剂的抑制。磷酸二酯酶抑制剂（异丁基甲基黄嘌呤）、腺苷酸环化酶激活剂（福斯高林）和cAMP的膜渗透性类似物（8-溴-cAMP），导致许多幼虫从接种区域向其他区域移动。据我们所知，这是关于彭亨布鲁线虫幼虫对宿主血清趋化反应的首次报道。我们得出结论，彭亨布鲁线虫幼虫对宿主血清表现出趋化反应，并且cAMP和cAMP依赖性蛋白激酶参与信号转导。
