de Bedout Catalina, Ayabaca Julio, Vega Ricardo, Méndez Matilde, Santiago Axel R, Pabón María Lucrecia, Tabares Angela, Arango Myrtha, Restrepo Angela, Newell Vance
Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia.
Biomedica. 2003 Mar;23(1):31-7.
Infections caused by yeasts belonging to the genus Candida have increased dramatically in the last decades, especially in hospital settings. Concomittantly, antimycotic resistance has emerged, as well as the appearance of non-Candida albicans isolates. To standardize in vitro antifungal susceptibility tests, the agar diffusion test was developed using disks impregnated with the antimycotic compound. Electronic recording of the inhibition zone (BIOMIC), furnishes objective values for the minimal inhibitory concentration (MIC). The fluconazole susceptibility patterns were determined for Candida species isolated from 2.139 patients seen in outpatient clinics or in health-care centers in Colombia, Ecuador and Venezuela. Candida albicans was the species most frequently isolated (62%), followed at a distance by Candida parapsilosis (11%), Candida tropicalis (8.5%), Candida glabata (3.5%) and Candida krusei (2.2%). MIC determinations showed that 88.1% of these isolates were susceptible to fluconazole, 5.1% were susceptible-dose-dependant and 6.8% resistant. An important proportion (92.1%) of the C. albicans isolates proved susceptible while resistance predominated in the remaining species. These results indicate that the BIOMIC method is rapid and simple, constituting a suitable tool for the epidemiologic surveillance of resistance in Candida species.
在过去几十年中，由念珠菌属酵母菌引起的感染显著增加，尤其是在医院环境中。与此同时，出现了抗真菌耐药性，以及非白色念珠菌分离株。为了标准化体外抗真菌药敏试验，开发了使用浸渍有抗真菌化合物的纸片的琼脂扩散试验。抑制圈的电子记录（BIOMIC）为最低抑菌浓度（MIC）提供了客观值。测定了从哥伦比亚、厄瓜多尔和委内瑞拉的门诊诊所或医疗中心的2139名患者中分离出的念珠菌属的氟康唑药敏模式。白色念珠菌是最常分离出的菌种（62%），其次是近平滑念珠菌（11%）、热带念珠菌（8.5%）、光滑念珠菌（3.5%）和克柔念珠菌（2.2%）。MIC测定表明，这些分离株中88.1%对氟康唑敏感，5.1%为剂量依赖性敏感，6.8%耐药。很大比例（92.1%）的白色念珠菌分离株被证明敏感，而耐药性在其余菌种中占主导。这些结果表明，BIOMIC方法快速简单，是念珠菌属耐药性流行病学监测的合适工具。
