Obana Hirotaka, Okihashi Masahiro, Akutsu Kazuhiko, Kitagawa Yoko, Hori Shinjiro
Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari, Osaka 5370025, Japan.
J Agric Food Chem. 2003 Apr 23;51(9):2501-5. doi: 10.1021/jf0261102.
A rapid and simple extraction method for the simultaneous analysis of five neonicotinoid insecticides has been developed. Twelve different fruit and vegetable matrixes were extracted with methanol and cleaned up using a graphitized carbon solid phase extraction cartridge loading with a 20% methanol solution. The concentrated eluate after methanol elution was then analyzed for pesticide residues by liquid chromatography/mass spectrometry in the APCI positive mode. The five pesticides including nitenpyram, thiamethoxam, imidacloprid, acetamiprid, and thiacloprid were recovered at 70-95% at spike levels of 0.1 and 1 mg/kg in bell pepper, cucumber, eggplant, grape, grapefruit, Japanese radish, peach, pear, potato, rice, and tomato. Relative standard deviations were less than 10% for all of the recovery tests. The proposed method is fast, easy to perform, and could be utilized for regular monitoring of pesticide residues.
已开发出一种快速简便的提取方法，用于同时分析五种新烟碱类杀虫剂。用甲醇提取十二种不同的果蔬基质，并用装填20%甲醇溶液的石墨化碳固相萃取柱进行净化。甲醇洗脱后的浓缩洗脱液然后通过液相色谱/质谱在大气压化学电离正模式下分析农药残留。在甜椒、黄瓜、茄子、葡萄、柚子、白萝卜、桃子、梨、土豆、大米和番茄中，啶虫脒、噻虫嗪、吡虫啉、啶虫脒和噻虫啉这五种农药在0.1和1 mg/kg的加标水平下回收率为70-95%。所有回收率测试的相对标准偏差均小于10%。所提出的方法快速、易于操作，可用于农药残留的常规监测。
