Suzuki Kazutaka, Hirai Hirofumi, Murata Hitoshi, Nishida Tomoaki
Department of Forest Resources Science, Faculty of Agriculture, Shizuoka University, Ohya 836, Japan.
Water Res. 2003 Apr;37(8):1972-5. doi: 10.1016/S0043-1354(02)00533-X.
We investigated whether manganese peroxidase (MnP) and the laccase-mediator system with 1-hydroxybenzotriazole (HBT) as mediator can remove the estrogenic activities of the steroidal hormones 17beta-estradiol (E(2)) and ethinylestradiol (EE(2)). Using the yeast two-hybrid assay system, we confirmed that the estrogenic activities of E(2) and EE(2) are much higher than those of bisphenol A and nonylphenol. Greater than 80% of the estrogenic activities of E(2) and EE(2) were removed following 1-h treatment with MnP or the laccase-HBT system; extending the treatment time to 8h removed the remaining estrogenic activity of both steroidal hormones. HPLC analysis demonstrated that E(2) and EE(2) had disappeared almost completely in the reaction mixture after a 1-h treatment. These results strongly suggest that these ligninolytic enzymes are effective in removing the estrogenic activities of E(2) and EE(2).
我们研究了锰过氧化物酶（MnP）以及以1-羟基苯并三唑（HBT）为介质的漆酶-介质系统是否能够去除甾体激素17β-雌二醇（E₂）和乙炔雌二醇（EE₂）的雌激素活性。通过酵母双杂交检测系统，我们证实E₂和EE₂的雌激素活性远高于双酚A和壬基酚。用MnP或漆酶-HBT系统处理1小时后，E₂和EE₂超过80%的雌激素活性被去除；将处理时间延长至8小时，则可去除这两种甾体激素剩余的雌激素活性。高效液相色谱分析表明，处理1小时后，反应混合物中的E₂和EE₂几乎完全消失。这些结果有力地表明，这些木质素降解酶在去除E₂和EE₂的雌激素活性方面是有效的。
