Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
Curr Microbiol. 2013 Jun;66(6):544-7. doi: 10.1007/s00284-013-0310-0. Epub 2013 Jan 20.
Bisphenol A (BPA) was treated with hyper lignin-degrading fungus Phanerochaete sordida YK-624 under ligninolytic condition. After preculturing P. sordida YK-624 for 4 days, BPA (final concentration, 1 and 0.1 mM) was added to cultures. Both 1- and 0.1-mM BPA were effectively decreased within a 24-h treatment and two metabolites were detected. Two metabolites (5,5'-bis-[1-(4-hydroxy-phenyl)1-methyl-ethyl]-biphenyl-2,2'-diol and 4-(2-(4-hydroxy-phenyl) propan-2-yl)-2-(4-(2-(4-hydroxyphenyl) propan-2-yl) phenoxy)phenol) were identified by ESI-MS and NMR analysis. These results indicated that BPA was oxidized to BPA phenoxy radicals by ligninolytic enzymes and then dimerized at extracellular region.
双酚 A (BPA) 在木质素降解真菌密粘褶菌 YK-624 存在的木质素降解条件下进行处理。在密粘褶菌 YK-624 预培养 4 天后，将 BPA（终浓度为 1 和 0.1 mM）加入培养物中。在 24 小时的处理时间内，1 mM 和 0.1 mM 的 BPA 均被有效降解，并且检测到两种代谢产物。通过 ESI-MS 和 NMR 分析鉴定出两种代谢产物（5,5'-双-[1-(4-羟基苯基)-1-甲基乙基]-联苯-2,2'-二醇和 4-(2-(4-羟基苯基)丙-2-基)-2-(4-(2-(4-羟基苯基)丙-2-基)苯氧基)苯酚）。这些结果表明，BPA 被木质素降解酶氧化成 BPA 酚氧自由基，然后在细胞外区域二聚化。
