通过在G带和R带染色体上对23个染色体特异性基因进行荧光原位杂交定位，建立R带兔核型命名法。

Establishment of an R-banded rabbit karyotype nomenclature by FISH localization of 23 chromosome-specific genes on both G- and R-banded chromosomes.

作者信息

Hayes H, Rogel-Gaillard C, Zijlstra C, De Haan N A, Urien C, Bourgeaux N, Bertaud M, Bosma A A

机构信息

Laboratoire de Génétique biochimique et Cytogénétique, INRA, Jouy-en-Josas, France.

出版信息

Cytogenet Genome Res. 2002;98(2-3):199-205. doi: 10.1159/000069807.

DOI:10.1159/000069807

PMID:12698004

Abstract

Direct detection of fluorescent in situ hybridization signals on R-banded chromosomes stained with propidium iodide is a rapid and efficient method for constructing cytogenetic maps for species with R-banded standard karyotypes. In this paper, our aim is to establish an R-banded rabbit karyotype nomenclature that is in total agreement with the 1981 G-banded standard nomenclature. For this purpose, we have produced new GTG- and RBG-banded mid-metaphase karyotypes and an updated version of ideograms of R-banded rabbit chromosomes. In addition, to confirm correlations between G- and R-banded chromosomes, we have defined a set of 23 rabbit BAC clones, each containing a specific gene, one marker gene per rabbit chromosome, and we have localized precisely each BAC clone by FISH on both G- and R-banded chromosomes.

摘要

在碘化丙啶染色的R带染色体上直接检测荧光原位杂交信号，是为具有R带标准核型的物种构建细胞遗传图谱的一种快速有效的方法。在本文中，我们的目的是建立一种与1981年G带标准命名完全一致的R带兔核型命名法。为此，我们制作了新的GTG和RBG带中期核型以及R带兔染色体 ideogram 的更新版本。此外，为了确认G带和R带染色体之间的相关性，我们定义了一组23个兔BAC克隆，每个克隆包含一个特定基因，即每条兔染色体一个标记基因，并且我们通过荧光原位杂交在G带和R带染色体上精确地定位了每个BAC克隆。