Raudsepp T, Christensen K, Chowdhar B P
Division of Animal Genetics, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Chromosome Res. 2000;8(8):659-70. doi: 10.1023/a:1026707002538.
With the expansion of comparative genome analysis across different mammals, there is an increasing need to have well-defined banded karyotypes for the species chosen for investigation. In this context, the steadily growing gene mapping data in the donkey urgently require a framework whereby alignment/comparison of genetic information can be readily made with equids and other mammalian species. Hence a GTG-banded karyotype of the donkey (Equus asinus; EAS) is presented, along with schematic drawings and nomenclature of the banded chromosomes. In addition, the most characteristic features of individual chromosomes are described and their relative size estimated. Using the FISH approach, the location of nucleolous organizer regions (NORs) and telomeric repeat sequences (TTAGGG) were detected. Where possible, information on asine chromosomes is supplemented with known/likely equine and human homologues. The study thus primarily aims to provide an appropriate cytogenetic basis for the donkey chromosomes, so that research focused on gene mapping and comparative genomics in this species can be reported under a common format.
随着跨不同哺乳动物的比较基因组分析不断扩展，对于所选用于研究的物种，越来越需要有明确界定的带型核型。在此背景下，驴中不断增长的基因图谱数据迫切需要一个框架，以便能够轻松地与马科动物及其他哺乳动物物种进行遗传信息的比对/比较。因此，本文呈现了驴（Equus asinus；EAS）的GTG带型核型，以及带型染色体的示意图和命名法。此外，还描述了各条染色体最具特征性的特征，并估计了它们的相对大小。使用荧光原位杂交（FISH）方法，检测了核仁组织区（NORs）和端粒重复序列（TTAGGG）的位置。在可能的情况下，关于驴染色体的信息会补充已知/可能的马和人类同源染色体信息。因此，本研究主要旨在为驴染色体提供合适的细胞遗传学基础，以便能够以通用格式报告针对该物种的基因图谱和比较基因组学研究。
