粗糙脉孢菌核糖核酸的核苷酸组成
NUCLEOTIDE COMPOSITION OF RIBONUCLEIC ACID FROM NEUROSPORA CRASSA.
作者信息
HENNEY H, STORCK R
出版信息
J Bacteriol. 1963 Apr;85(4):822-6. doi: 10.1128/jb.85.4.822-826.1963.
Henney, H. (The University of Texas, Austin) and R. Storck. Nucleotide composition of ribonucleic acid from Neurospora crassa. J. Bacteriol. 85:822-826. 1963.-The nucleotide composition of total, ribosomal, and soluble ribonucleic acid (RNA) from the conidia and the hyphae of Neurospora crassa was determined. The corresponding RNA classes from the two morphological types had the same base ratio. Total, ribosomal, and soluble RNA from hyphae contained, respectively, 51.0, 50.2, and 61.6% guanylic acid (G) plus cytidylic acid (C), and from conidia, 50.6, 49.9, and 62.1%. The proportion of nucleotides in ribosomal RNA was in close agreement with that reported for ribosomal RNA from other organisms. Soluble RNA contained 2.8% pseudouridylic acid, 36.0% C, and 25.5% G, and differed from soluble RNA from other sources in its high C content. Centrifugation of purified RNA from conidia and hyphae, through a linear sucrose-density gradient, yielded identical sedimentation profiles.
亨尼，H.（得克萨斯大学奥斯汀分校）和R.斯托克。粗糙脉孢菌核糖核酸的核苷酸组成。《细菌学杂志》85:822 - 826。1963年。——测定了粗糙脉孢菌分生孢子和菌丝体中总核糖核酸、核糖体核糖核酸和可溶性核糖核酸（RNA）的核苷酸组成。两种形态类型的相应RNA类别具有相同的碱基比率。菌丝体中的总RNA、核糖体RNA和可溶性RNA分别含有51.0%、50.2%和61.6%的鸟苷酸（G）加胞苷酸（C），分生孢子中的分别为50.6%、49.9%和62.1%。核糖体RNA中核苷酸的比例与其他生物核糖体RNA的报道比例密切一致。可溶性RNA含有2.8%的假尿苷酸、36.0%的C和25.5%的G，并且其高C含量与其他来源的可溶性RNA不同。通过线性蔗糖密度梯度对分生孢子和菌丝体的纯化RNA进行离心，得到了相同的沉降图谱。
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