Simon P L, Farrar J J, Kind P D
J Immunol. 1977 Apr;118(4):1129-31.
A supernatant from human mixed leukocyte cultures (MLC), Xenogeneic Reconstitution Factor (XRF), was added to one-way murine MLC. This supernatant greatly enhances the generation of cytotoxic cells from C57BL/6 or DBA/2 responder thymocytes, as assayed by a standard 51Cr-release assay. This enhancement is shown 1) to be dependent upon the presence of BDF1 semi-allogeneic stimulator cells and 2) to result in the generation of cytotoxic cells that are specific for the H-2 type of the stimulating alloantigen. In some experiments, alloantigen-stimulated murine spleen cells, when cultured with XRF showed increased induction of cell-mediated cytotoxicity. These experiments show that XRF contains a cytotoxic-cell activating factor(s) that is functionally similar to those found in murine-derived supernatants, and that this factor can participate in the generation of cytotoxic cells from precursors present in the murine thymus.
将人混合白细胞培养物（MLC）的上清液，即异种重建因子（XRF），添加到单向小鼠MLC中。通过标准的51Cr释放试验检测发现，该上清液能极大地增强C57BL/6或DBA/2反应性胸腺细胞产生细胞毒性细胞的能力。这种增强作用表现为：1）依赖于BDF1半同种异体刺激细胞的存在；2）导致产生对刺激同种异体抗原的H-2类型具有特异性的细胞毒性细胞。在一些实验中，同种异体抗原刺激的小鼠脾细胞与XRF一起培养时，显示出细胞介导的细胞毒性诱导增加。这些实验表明，XRF含有一种细胞毒性细胞激活因子，其功能与在小鼠来源的上清液中发现的因子相似，并且该因子可以参与从小鼠胸腺中存在的前体细胞产生细胞毒性细胞的过程。
