Okami Kenji, Imate Yuji, Hashimoto Yoko, Kamada Tomomi, Takahashi Masahiro
Department of Otolaryngology, Tokai University, School of Medicine, Isehara, Kanagawa, Japan.
Tokai J Exp Clin Med. 2002 Sep;27(3):85-9.
A key to improve the prognosis of head and neck cancers is an early diagnosis of the disease. No screening method to detect these cancers has been developed yet. Molecular techniques using polymerase chain reaction are a sensitive method to detect a small population of cancer cells among normal cells. We conducted a series of microsatellite analysis to detect cancer cells in saliva from 23 oral and pharyngeal cancer patients. Eight microsatellite markers were selected to test for microsatellite instability (MSI) in the tumor and saliva samples. Of 23 samples, 5 (22%) had MSI in the tumor samples. In 4 of 5 (80%) MSI positive samples, we detected the identical MSI in saliva. The possibility of the molecular screening and molecular follow-up is discussed.
改善头颈癌预后的关键在于对该疾病的早期诊断。目前尚未开发出检测这些癌症的筛查方法。使用聚合酶链反应的分子技术是在正常细胞中检测少量癌细胞的灵敏方法。我们进行了一系列微卫星分析，以检测23例口腔和咽癌患者唾液中的癌细胞。选择了8个微卫星标记来检测肿瘤和唾液样本中的微卫星不稳定性（MSI）。在23个样本中，5个（22%）肿瘤样本存在MSI。在5个MSI阳性样本中的4个（80%）中，我们在唾液中检测到相同的MSI。文中讨论了分子筛查和分子随访的可能性。
