Azeredo L, Pacheco A P, Lopes I, Oliveira R, Vieira M J
Centro de Engenharia Biológica-IBQF, Universidade do Minho, 4710-057 Braga, Portugal.
Water Sci Technol. 2003;47(5):77-82.
The efficacy of the surfactants SDS and CTAB in detaching P. fluorescens from glass surface was evaluated in a parallel plate flow chamber. This device enables "in situ" determinations of cells detachment following the application of surfactants under well controlled hydrodynamic conditions. The results showed that SDS was able to remove almost all adhering bacteria in a short period of time, whereas CTAB did not promote much cell desorption. On the contrary, this surfactant increased the adhesion strength between cells and glass. Both surfactants promoted different alterations of cell surface properties, which explain their dissimilar effectiveness as cleansing agents.
在平行平板流动腔中评估了表面活性剂十二烷基硫酸钠（SDS）和十六烷基三甲基溴化铵（CTAB）从玻璃表面分离荧光假单胞菌的效果。该装置能够在良好控制的流体动力学条件下施加表面活性剂后“原位”测定细胞的分离情况。结果表明，SDS能够在短时间内去除几乎所有附着的细菌，而CTAB并没有促进太多细胞解吸。相反，这种表面活性剂增加了细胞与玻璃之间的粘附强度。两种表面活性剂都促进了细胞表面性质的不同改变，这解释了它们作为清洁剂的不同效果。
