Garrett Trevor Roger, Bhakoo Manmohan, Zhang Zhibing
Centre for Formulation Engineering, Chemical Engineering, School of Engineering, The University of Birmingham, Edgbaston, Birmingham, UK.
Biotechnol Lett. 2008 Mar;30(3):427-33. doi: 10.1007/s10529-007-9580-6. Epub 2007 Oct 31.
Flow device analyses and micromanipulation were used to assess the adhesive and cohesive integrity of the immobilised bacterial populations (biomass) of Pseudomonas fluorescens, which were harvested at different growth times and applied to a substrate made of stainless steel subsequently accommodated in a specially designed flow chamber. After the biomass was exposed to a fluidic environment for a period of time, the biomass samples were removed from the flow chamber and the apparent adhesion and cohesion of the remaining biomass was measured using a micromanipulation technique. The surface area of the substrate covered by the biomass exposed to the fluid flow was monitored by a digital camera and then quantified by image analysis. The results indicate a strong correlation between micromanipulation measurements and flow chamber experiments. The micromanipulation data show that the apparent adhesive strength of the biomass increased with the growth time. Moreover, the apparent adhesive strength was found to be stronger than the bacterial cohesive strength. The data was used to interpret the removal behaviour of the biomass from the flow chamber. Using these techniques, specific mechanisms of biomass detachment from a surface and optimised cleaning strategies can be postulated.
采用流动装置分析和显微操作来评估荧光假单胞菌固定化细菌群体（生物量）的粘附和内聚完整性，这些细菌群体在不同生长时间收获，随后施加到由不锈钢制成的、放置在专门设计的流动腔室中的底物上。在生物量暴露于流体环境一段时间后，将生物量样品从流动腔室中取出，并使用显微操作技术测量剩余生物量的表观粘附力和内聚力。通过数码相机监测暴露于流体流动的生物量覆盖的底物表面积，然后通过图像分析进行量化。结果表明显微操作测量与流动腔室实验之间存在很强的相关性。显微操作数据表明，生物量的表观粘附强度随生长时间增加。此外，发现表观粘附强度强于细菌内聚强度。这些数据用于解释生物量从流动腔室中的去除行为。利用这些技术，可以推测生物量从表面脱离的具体机制以及优化的清洁策略。
