Latouche G Nicolas, Sorrell Tania C, Meyer Wieland
Molecular Mycology Laboratory, CIDM, Westmead Hospital, ICPMR, Level 3 Room 3114A, Darcy Road, NSW 2145, Australia.
FEMS Yeast Res. 2002 Dec;2(4):551-61. doi: 10.1111/j.1567-1364.2002.tb00122.x.
Cryptococcus neoformans var. gattii (serotypes B and C) is a human pathogen, ecologically, biochemically, clinically and genetically different from C. neoformans var. grubii (serotype A) and C. neoformans var. neoformans (serotype D). The phospholipase B (PLB1) gene from serotypes B and C was isolated and characterised. It resembled the serotype A and D genes, with an overall sequence homology of more than 85%. The respective open reading frames were 2236 bp (serotype B) and 2239 bp (serotype C) in length. Each contained six introns and encoded a 68-kDa protein destined for secretion. PLB1 was located on the second smallest chromosome in both serotypes. Gene expression, measured as mRNA, was not regulated by temperature, pH or exogenous nutrients.
