Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 43421, Caixa Postal 15005, Porto Alegre, RS 91501-970, Brazil.
J Proteome Res. 2012 Jan 1;11(1):189-205. doi: 10.1021/pr2005296. Epub 2011 Oct 26.
Iron is essential and ubiquitous in living organisms. The competition for this micronutrient between the host and its pathogens has been related to disease establishment. Cryptococcus gattii is an encapsulated yeast that causes cryptococcosis mainly in immunocompetent individuals. In this study, we analyzed the proteomic profile of the C. gattii R265 Vancouver Island isolate under iron-depleted and -repleted conditions by multidimensional protein identification technology (MudPIT) and by 2D-GE. Proteins and key mechanisms affected by alteration of iron levels such as capsule production, cAMP-signaling pathway, response to stress, and metabolic pathways related to mitochondrial function were identified. Our results also show both proteomic methodologies employed to be complementary.
铁是生命体中必需的且无处不在的元素。宿主与其病原体对这种微量元素的竞争与疾病的发生有关。新生隐球菌是一种包裹的酵母，主要在免疫功能正常的个体中引起隐球菌病。在这项研究中，我们通过多维蛋白质鉴定技术（MudPIT）和 2D-GE 分析了铁耗竭和铁补充条件下 C. gattii R265 温哥华岛分离株的蛋白质组图谱。鉴定到了受铁水平变化影响的蛋白质和关键机制，如荚膜产生、cAMP 信号通路、应激反应以及与线粒体功能相关的代谢途径。我们的结果还表明，所采用的两种蛋白质组学方法是互补的。
