铁可用性对荚膜组织胞浆菌影响的蛋白质组学分析。
Proteomic profiling of the influence of iron availability on Cryptococcus gattii.
机构信息
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 43421, Caixa Postal 15005, Porto Alegre, RS 91501-970, Brazil.
出版信息
J Proteome Res. 2012 Jan 1;11(1):189-205. doi: 10.1021/pr2005296. Epub 2011 Oct 26.
Abstract
Iron is essential and ubiquitous in living organisms. The competition for this micronutrient between the host and its pathogens has been related to disease establishment. Cryptococcus gattii is an encapsulated yeast that causes cryptococcosis mainly in immunocompetent individuals. In this study, we analyzed the proteomic profile of the C. gattii R265 Vancouver Island isolate under iron-depleted and -repleted conditions by multidimensional protein identification technology (MudPIT) and by 2D-GE. Proteins and key mechanisms affected by alteration of iron levels such as capsule production, cAMP-signaling pathway, response to stress, and metabolic pathways related to mitochondrial function were identified. Our results also show both proteomic methodologies employed to be complementary.
摘要
铁是生命体中必需的且无处不在的元素。宿主与其病原体对这种微量元素的竞争与疾病的发生有关。新生隐球菌是一种包裹的酵母，主要在免疫功能正常的个体中引起隐球菌病。在这项研究中，我们通过多维蛋白质鉴定技术（MudPIT）和 2D-GE 分析了铁耗竭和铁补充条件下 C. gattii R265 温哥华岛分离株的蛋白质组图谱。鉴定到了受铁水平变化影响的蛋白质和关键机制，如荚膜产生、cAMP 信号通路、应激反应以及与线粒体功能相关的代谢途径。我们的结果还表明，所采用的两种蛋白质组学方法是互补的。
相似文献
1
Proteomic profiling of the influence of iron availability on Cryptococcus gattii.铁可用性对荚膜组织胞浆菌影响的蛋白质组学分析。
J Proteome Res. 2012 Jan 1;11(1):189-205. doi: 10.1021/pr2005296. Epub 2011 Oct 26.
2
Chitosan Biosynthesis and Virulence in the Human Fungal Pathogen Cryptococcus gattii.壳聚糖生物合成与人类真菌病原体荚膜组织胞浆菌的毒力。
mSphere. 2019 Oct 9;4(5):e00644-19. doi: 10.1128/mSphere.00644-19.
3
Proteomic profile of Cryptococcus gattii biofilm: Metabolic shift and the potential activation of electron chain transport.荚膜组织胞浆菌生物膜的蛋白质组学特征：代谢转变和电子链传递的潜在激活。
J Proteomics. 2024 Jan 6;290:105022. doi: 10.1016/j.jprot.2023.105022. Epub 2023 Oct 12.
4
Identification of genes expressed by Cryptococcus gattii during iron deprivation.鉴定加氏隐球菌在缺铁条件下表达的基因。
Braz J Microbiol. 2014 Oct 9;45(3):813-20. doi: 10.1590/s1517-83822014000300008. eCollection 2014.
5
Congenic strains for genetic analysis of virulence traits in Cryptococcus gattii.用于分析荚膜组织胞浆菌毒力特征的基因分析同源系。
Infect Immun. 2013 Jul;81(7):2616-25. doi: 10.1128/IAI.00018-13. Epub 2013 May 13.
6
Cryptococcus neoformans and Cryptococcus gattii genes preferentially expressed during rat macrophage infection.新生隐球菌和格特隐球菌在大鼠巨噬细胞感染过程中优先表达的基因。
Med Mycol. 2010 Nov;48(7):932-41. doi: 10.3109/13693781003677494.
7
Time-course proteome analysis reveals the dynamic response of Cryptococcus gattii cells to fluconazole.时间进程蛋白质组学分析揭示了隐球菌属细胞对氟康唑的动态反应。
PLoS One. 2012;7(8):e42835. doi: 10.1371/journal.pone.0042835. Epub 2012 Aug 6.
8
Immunoproteomics and immunoinformatics analysis of Cryptococcus gattii: novel candidate antigens for diagnosis.荚膜组织胞浆菌免疫蛋白质组学和免疫信息学分析：新型诊断候选抗原。
Future Microbiol. 2013 Apr;8(4):549-63. doi: 10.2217/fmb.13.22.
9
Transcriptional Heterogeneity of VGII Compared with Non-VGII Lineages Underpins Key Pathogenicity Pathways.与非 VGII 谱系相比，VGII 的转录异质性为关键致病途径提供了基础。
mSphere. 2018 Oct 24;3(5):e00445-18. doi: 10.1128/mSphere.00445-18.
10
Cryptococcus gattii virulence composite: candidate genes revealed by microarray analysis of high and less virulent Vancouver island outbreak strains.荚膜组织胞浆菌毒力复合群：高毒力和低毒力温哥华岛暴发株的芯片分析揭示的候选基因。
PLoS One. 2011 Jan 13;6(1):e16076. doi: 10.1371/journal.pone.0016076.
引用本文的文献
1
Corrected and republished from: "Extracellular Vesicle Formation in Impacts Fungal Virulence".校正后重新发布自：“细胞外囊泡形成影响真菌毒力”。
Infect Immun. 2024 Apr 9;92(4):e0003724. doi: 10.1128/iai.00037-24. Epub 2024 Mar 12.
2
A J Domain Protein Functions as a Histone Chaperone to Maintain Genome Integrity and the Response to DNA Damage in a Human Fungal Pathogen.一个 J 结构域蛋白作为组蛋白伴侣，维持人类真菌病原体的基因组完整性和对 DNA 损伤的反应。
mBio. 2021 Dec 21;12(6):e0327321. doi: 10.1128/mbio.03273-21.
3
Identification of Disease-Associated Cryptococcal Proteins Reactive With Serum IgG From Cryptococcal Meningitis Patients.鉴定与新型隐球菌性脑膜炎患者血清 IgG 反应的疾病相关隐球菌蛋白。
Front Immunol. 2021 Jul 23;12:709695. doi: 10.3389/fimmu.2021.709695. eCollection 2021.
4
Characterization of the Potential Probiotic sp. V33 Antagonizing Based on Iron Competition.基于铁竞争对潜在益生菌V33菌株的特性分析
Iran J Biotechnol. 2020 Jan 1;18(1):e2259. doi: 10.30498/IJB.2019.85192. eCollection 2020 Jan.
5
The Novel J-Domain Protein Mrj1 Is Required for Mitochondrial Respiration and Virulence in Cryptococcus neoformans.新型 J 结构域蛋白 Mrj1 是新型隐球菌线粒体呼吸和毒力所必需的。
mBio. 2020 Jun 9;11(3):e01127-20. doi: 10.1128/mBio.01127-20.
6
Indole contributes to tetracycline resistance via the outer membrane protein OmpN in Vibrio splendidus.吲哚通过灿烂弧菌外膜蛋白 OmpN 促进四环素耐药性。
World J Microbiol Biotechnol. 2020 Feb 22;36(3):36. doi: 10.1007/s11274-020-02813-6.
7
Analysis of the Protein Kinase A-Regulated Proteome of Cryptococcus neoformans Identifies a Role for the Ubiquitin-Proteasome Pathway in Capsule Formation.新型隐球菌蛋白激酶A调控蛋白质组分析确定泛素-蛋白酶体途径在荚膜形成中的作用。
mBio. 2016 Jan 12;7(1):e01862-15. doi: 10.1128/mBio.01862-15.
8
Identification of genes expressed by Cryptococcus gattii during iron deprivation.鉴定加氏隐球菌在缺铁条件下表达的基因。
Braz J Microbiol. 2014 Oct 9;45(3):813-20. doi: 10.1590/s1517-83822014000300008. eCollection 2014.
9
The influence of iron on the proteomic profile of Chromobacterium violaceum.铁对紫色色杆菌蛋白质组图谱的影响。
BMC Microbiol. 2014 Oct 20;14:267. doi: 10.1186/s12866-014-0267-6.
10
Conflicting interests in the pathogen-host tug of war: fungal micronutrient scavenging versus mammalian nutritional immunity.病原体与宿主拔河比赛中的利益冲突：真菌微量营养素掠夺与哺乳动物营养免疫
PLoS Pathog. 2014 Mar 13;10(3):e1003910. doi: 10.1371/journal.ppat.1003910. eCollection 2014 Mar.
本文引用的文献
1
An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database.一种将肽的串联质谱数据与蛋白质数据库中氨基酸序列相关联的方法。
J Am Soc Mass Spectrom. 1994 Nov;5(11):976-89. doi: 10.1016/1044-0305(94)80016-2.
2
Silver Staining, Digestion, and Extraction of Peptides from an Acrylamide Gel for MS Analysis.银染、消化以及从丙烯酰胺凝胶中提取肽段用于质谱分析
CSH Protoc. 2006 Dec 1;2006(7):pdb.prot4661. doi: 10.1101/pdb.prot4661.
3
The homeostasis of iron, copper, and zinc in paracoccidioides brasiliensis, cryptococcus neoformans var. Grubii, and cryptococcus gattii: a comparative analysis.巴西副球孢子菌、新生隐球菌 var. Grubii 和格特隐球菌中铁、铜和锌的体内平衡：比较分析。
Front Microbiol. 2011 Mar 21;2:49. doi: 10.3389/fmicb.2011.00049. eCollection 2011.
4
Expanding fungal pathogenesis: Cryptococcus breaks out of the opportunistic box.拓展真菌发病机制：新型隐球菌打破机会主义框框。
Nat Rev Microbiol. 2011 Mar;9(3):193-203. doi: 10.1038/nrmicro2522.
5
Genome variation in Cryptococcus gattii, an emerging pathogen of immunocompetent hosts.新型隐球菌（Cryptococcus gattii）的基因组变异，这种病原体能感染免疫功能正常的宿主。
mBio. 2011 Feb 8;2(1):e00342-10. doi: 10.1128/mBio.00342-10. Print 2011.
6
Cryptococcus gattii virulence composite: candidate genes revealed by microarray analysis of high and less virulent Vancouver island outbreak strains.荚膜组织胞浆菌毒力复合群：高毒力和低毒力温哥华岛暴发株的芯片分析揭示的候选基因。
PLoS One. 2011 Jan 13;6(1):e16076. doi: 10.1371/journal.pone.0016076.
7
Cryptococcosis: update and emergence of Cryptococcus gattii.隐球菌病：加氏隐球菌的最新情况及出现
Vet Clin Pathol. 2011 Mar;40(1):4-17. doi: 10.1111/j.1939-165X.2010.00281.x. Epub 2011 Jan 18.
8
The role of iron in the immune response to bacterial infection.铁元素在细菌感染免疫反应中的作用。
Immunol Res. 2011 May;50(1):1-9. doi: 10.1007/s12026-010-8199-1.
9
HapX positively and negatively regulates the transcriptional response to iron deprivation in Cryptococcus neoformans.HapX 正向和负向调控新生隐球菌中铁饥饿诱导的转录反应。
PLoS Pathog. 2010 Nov 24;6(11):e1001209. doi: 10.1371/journal.ppat.1001209.
10
The iron-responsive, GATA-type transcription factor Cir1 influences mating in Cryptococcus neoformans.铁反应性、GATA 型转录因子 Cir1 影响新型隐球菌的交配。
Mol Cells. 2011 Jan;31(1):73-7. doi: 10.1007/s10059-011-0011-0. Epub 2010 Nov 25.
Zotero 插件