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蜂胶醇提物载药尼欧斯omes 可降低巨噬细胞中磷脂酶 B1、生物膜形成和内复制。

Ethanolic Extract Propolis-Loaded Niosomes Diminish Phospholipase B1, Biofilm Formation, and Intracellular Replication of in Macrophages.

机构信息

Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.

Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.

出版信息

Molecules. 2023 Aug 24;28(17):6224. doi: 10.3390/molecules28176224.

DOI:10.3390/molecules28176224
PMID:37687052
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10488685/
Abstract

Secretory phospholipase B1 (PLB1) and biofilms act as microbial virulence factors and play an important role in pulmonary cryptococcosis. This study aims to formulate the ethanolic extract of propolis-loaded niosomes (Nio-EEP) and evaluate the biological activities occurring during PLB1 production and biofilm formation of . Some physicochemical characterizations of niosomes include a mean diameter of 270 nm in a spherical shape, a zeta-potential of -10.54 ± 1.37 mV, and 88.13 ± 0.01% entrapment efficiency. Nio-EEP can release EEP in a sustained manner and retains consistent physicochemical properties for a month. Nio-EEP has the capability to permeate the cellular membranes of , causing a significant decrease in the mRNA expression level of . Interestingly, biofilm formation, biofilm thickness, and the expression level of biofilm-related genes ( and ) were also significantly reduced. Pre-treating with Nio-EEP prior to yeast infection reduced the intracellular replication of in alveolar macrophages by 47%. In conclusion, Nio-EEP mediates as an anti-virulence agent to inhibit PLB1 and biofilm production for preventing fungal colonization on lung epithelial cells and also decreases the intracellular replication of phagocytosed cryptococci. This nano-based EEP delivery might be a potential therapeutic strategy in the prophylaxis and treatment of pulmonary cryptococcosis in the future.

摘要

分泌型磷脂酶 B1 (PLB1) 和生物膜作为微生物毒力因子，在肺隐球菌病中发挥重要作用。本研究旨在制备载有蜂胶醇质体的尼欧体（Nio-EEP）并评估其在 PLB1 产生和生物膜形成过程中的生物学活性。尼欧体的一些理化特性包括平均直径为 270nm 的球形、-10.54±1.37mV 的 ζ 电位和 88.13±0.01%的包封效率。Nio-EEP 可以持续释放 EEP，并在一个月内保持一致的物理化学性质。Nio-EEP 能够穿透 的细胞膜，导致 的 mRNA 表达水平显著降低。有趣的是，生物膜形成、生物膜厚度和生物膜相关基因（ 和 ）的表达水平也显著降低。在酵母感染前用 Nio-EEP 预处理可使肺泡巨噬细胞内 的复制减少 47%。总之，Nio-EEP 作为一种抗毒力剂，可抑制 PLB1 和生物膜的产生，防止真菌在肺上皮细胞上定植，并降低吞噬的隐球菌的细胞内复制。这种基于纳米的 EEP 递药系统可能是未来预防和治疗肺隐球菌病的一种有潜力的治疗策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7daa/10488685/698b5e5c3775/molecules-28-06224-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7daa/10488685/157cda4bc110/molecules-28-06224-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7daa/10488685/a7952f589b22/molecules-28-06224-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7daa/10488685/b989dd2c1d78/molecules-28-06224-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7daa/10488685/ee578634afdb/molecules-28-06224-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7daa/10488685/d649a27c480e/molecules-28-06224-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7daa/10488685/467471718b9b/molecules-28-06224-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7daa/10488685/672c5804fe0c/molecules-28-06224-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7daa/10488685/698b5e5c3775/molecules-28-06224-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7daa/10488685/157cda4bc110/molecules-28-06224-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7daa/10488685/a7952f589b22/molecules-28-06224-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7daa/10488685/b989dd2c1d78/molecules-28-06224-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7daa/10488685/ee578634afdb/molecules-28-06224-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7daa/10488685/d649a27c480e/molecules-28-06224-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7daa/10488685/467471718b9b/molecules-28-06224-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7daa/10488685/672c5804fe0c/molecules-28-06224-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7daa/10488685/698b5e5c3775/molecules-28-06224-g008.jpg

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