Pearlstone J R, Carpenter M R, Smillie L B
J Biol Chem. 1977 Feb 10;252(3):971-7.
Troponin-T was cleaved by cyanogen bromide (CB) to produce the seven fragments CB1 (151 residues), CB3 (70 residues), CB2 (81 residues), CB5 (24 residues), CB4 (54 residues), CB7 (8 residues), and CB6 (21 residues). The NH2-terminal fragment CB1, composed of CB3 plus CB2, had an internal homoserine which was not completely cleaved. The amino acid sequence of CB2 was determined by a combination of automated and manual Edman degradation techniques. Peptides suitable for the latter method were derived from tryptic, alpha-chymotryptic, alpha-lytic protease, and thermolytic digestions. Fragment CB2 contains 81 of the 259 residues of troponin-T.
肌钙蛋白 - T 被溴化氰（CB）裂解产生七个片段：CB1（151 个残基）、CB3（70 个残基）、CB2（81 个残基）、CB5（24 个残基）、CB4（54 个残基）、CB7（8 个残基）和 CB6（21 个残基）。由 CB3 加 CB2 组成的 NH2 末端片段 CB1 有一个未被完全裂解的内部高丝氨酸。CB2 的氨基酸序列通过自动和手动 Edman 降解技术相结合的方法确定。适用于后一种方法的肽段来自胰蛋白酶、α - 胰凝乳蛋白酶、α - 溶细胞蛋白酶和嗜热菌蛋白酶消化产物。片段 CB2 包含肌钙蛋白 - T 的 259 个残基中的 81 个。
