Mahdavi Jafar, Borén Thomas, Vandenbroucke-Grauls Christina, Appelmelk Ben J
Department of Odontology/Oral Microbiology, Umeå University, 901 87 Umeå, Sweden.
Infect Immun. 2003 May;71(5):2876-80. doi: 10.1128/IAI.71.5.2876-2880.2003.
In vitro and in vivo studies from various groups have suggested that Helicobacter pylori lipopolysaccharide (LPS) Lewis x (Le(x)) antigens mediate bacterial adhesion. We have now reevaluated this hypothesis by studying the adherence in situ of H. pylori strain 11637 and its corresponding Le(x)-negative rfbM mutant to human gastric mucosa from patients (n = 22) with various gastric pathologies. Significant binding of the parent strain was observed in only 8 out of 22 sections; in four out of eight patients, the Le(x)-negative mutant bound less well. One of these four patients displayed no gastric abnormalities, and the other three showed dysplasia, metaplasia, and adenocarcinoma, respectively; hence, we are unable to define the circumstances under which LPS-mediated adhesion takes place. We conclude that H. pylori LPS plays a distinct but minor role in adhesion.
来自不同研究小组的体外和体内研究表明，幽门螺杆菌脂多糖（LPS）Lewis x（Le(x)）抗原介导细菌黏附。我们现在通过研究幽门螺杆菌菌株11637及其相应的Le(x)阴性rfbM突变体对患有各种胃部疾病的患者（n = 22）的人胃黏膜的原位黏附情况，重新评估了这一假设。在22个切片中，仅在8个切片中观察到亲本菌株的显著结合；在这8名患者中的4名患者中，Le(x)阴性突变体的结合情况较差。这4名患者中的1名没有胃部异常，另外3名分别表现为发育异常、化生和腺癌；因此，我们无法确定LPS介导黏附发生的情况。我们得出结论，幽门螺杆菌LPS在黏附中起明显但次要的作用。
