Lozniewski Alain, Haristoy Xavier, Rasko David A, Hatier Renée, Plénat François, Taylor Diane E, Angioi-Duprez Karine
Laboratoire de Bactériologie-Virologie, Faculté de Médecine, Université Henri-Poincaré, Vandoeuvre-les-Nancy, France. a/
Infect Immun. 2003 May;71(5):2902-6. doi: 10.1128/IAI.71.5.2902-2906.2003.
The role of Helicobacter pylori lipopolysaccharide (LPS) Lewis antigens in infection is still not well known. We investigated the influence of Lewis antigen expression by H. pylori on its internalization by AGS cells and the epithelium of human gastric xenografts in nude mice using isogenic mutants in LPS biosynthetic genes. In vivo, colonization rates were unaffected by the change in H. pylori Lewis antigen expression, whereas the number of viable intracellular bacteria was significantly higher with wild-type H. pylori strains expressing Lewis antigens when compared to the isogenic mutants in both models. H. pylori strains expressing more Lewis X antigens (Le(x)) were internalized at a higher rate than those expressing less Le(x), type II Lewis antigens (Le(a) or Le(b)) alone, or no Lewis antigens. Thus, Lewis antigens appear to be involved in the internalization of H. pylori by the gastric epithelium.
幽门螺杆菌脂多糖（LPS）Lewis抗原在感染中的作用仍未完全明确。我们利用LPS生物合成基因的同基因突变体，研究了幽门螺杆菌Lewis抗原表达对AGS细胞及裸鼠人胃异种移植上皮细胞摄取幽门螺杆菌的影响。在体内，幽门螺杆菌Lewis抗原表达的变化不影响其定植率，然而，在两种模型中，与同基因突变体相比，表达Lewis抗原的野生型幽门螺杆菌菌株的胞内活菌数显著更高。表达更多Lewis X抗原（Le(x)）的幽门螺杆菌菌株比表达较少Le(x)、单独表达II型Lewis抗原（Le(a)或Le(b)）或不表达Lewis抗原的菌株摄取率更高。因此，Lewis抗原似乎参与了胃上皮细胞对幽门螺杆菌的摄取。
