Moran A P, Sturegård E, Sjunnesson H, Wadström T, Hynes S O
Department of Microbiology, National University of Ireland, Galway.
FEMS Immunol Med Microbiol. 2000 Dec;29(4):263-70. doi: 10.1111/j.1574-695X.2000.tb01532.x.
The influence of lipopolysaccharide (LPS) O-polysaccharide chain production on the colonisation ability of Helicobacter pylori in four mouse models (NMRI, C57BL/6, CBA/Ca, and BALB/cA mice) was studied. H. pylori strains that produced smooth-form LPS (S-LPS) detectable in silver-stained electrophoretic gels colonised mice. In contrast, a laboratory-passaged strain G50 and the culture collection strain CCUG 17874 did not colonise mice; the former strain produced low amounts of O-chains only detectable in immunoblotting but not in silver-stained gels, whereas the latter produced rough-form LPS (R-LPS) without O-chains. Furthermore, a galE isogenic mutant, which produced R-LPS, did not colonise mice. However, after repeated broth culture, strains G50 and CCUG 17874 produced S-LPS detectable in silver-stained gels and were capable of colonising mice. Consistent with the production of O-chains, all colonising strains produced Lewis (Le) antigens, Le(x) and/or Le(y). Except for low expression of Le(y) by non-colonising G50, reflecting low production of O-chains, all other non-colonising strains and the galE mutant lacked expression of Le antigens consistent with their production of R-LPS. Lectin typing of strains supported these findings, and also showed that lectin types did not differ before and after colonisation. The low level of O-chain production and Le antigen expression by the non-colonising G50 may not be sufficient to aid colonisation. Examination of protein profiles of H. pylori strains before inoculation showed that protein expression was not significantly different between colonising and non-colonising strains. These results show that S-LPS production with O-chain expression is required by H. pylori for colonisation in a number of mouse models and that care should be taken with inoculating H. pylori strains that loss of O-chains does not occur during subculturing.
研究了脂多糖（LPS）O-多糖链产生对幽门螺杆菌在四种小鼠模型（NMRI、C57BL/6、CBA/Ca和BALB/cA小鼠）中定殖能力的影响。在银染电泳凝胶中可检测到产生光滑型LPS（S-LPS）的幽门螺杆菌菌株能定殖小鼠。相比之下，实验室传代菌株G50和保藏菌株CCUG 17874不能定殖小鼠；前一种菌株仅产生少量仅在免疫印迹中可检测到而在银染凝胶中检测不到的O链，而后一种产生无O链的粗糙型LPS（R-LPS）。此外，产生R-LPS的galE同基因突变体不能定殖小鼠。然而，经过反复肉汤培养后，菌株G50和CCUG 17874产生了在银染凝胶中可检测到的S-LPS，并能够定殖小鼠。与O链的产生一致，所有定殖菌株都产生了Lewis（Le）抗原Le(x)和/或Le(y)。除了非定殖菌株G50低表达Le(y)（反映O链产生量低）外，所有其他非定殖菌株和galE突变体均缺乏与它们产生R-LPS一致的Le抗原表达。菌株的凝集素分型支持了这些发现，并且还表明定殖前后凝集素类型没有差异。非定殖菌株G50的O链产生水平和Le抗原表达水平低可能不足以促进定殖。对接种前幽门螺杆菌菌株的蛋白质谱检查表明，定殖菌株和非定殖菌株之间的蛋白质表达没有显著差异。这些结果表明，幽门螺杆菌在多种小鼠模型中定殖需要产生带有O链表达的S-LPS，并且在接种幽门螺杆菌菌株时应注意传代培养过程中不会发生O链丢失。
