成人人类关节软骨的器官培养。II. 关节软骨基质各层中糖胺聚糖合成的差异率。
Organ culture of adult human articular cartilage. II. The differential rate of glycosaminoglycan synthesis in layers of articular cartilage matrix.
作者信息
Jacoby R K, Jayson M I
出版信息
J Rheumatol. 1975 Sep;2(3):280-6.
Adult human articular cartilage was maintained in culture for three days and labelled with 35SO4 for the first 48 hours. The explants were sectioned horizontally on the cryostat, and the sections from the top, middle, and deep layers analyzed separately. Autoradiographs were prepared from every twentieth section. The DNA content in each of the three layers was of the same order, but the sulfate uptake and uronic acid content were considerably reduced at the surface. It is suggested that the chondrocytes synthesize matrix appropriate to the layer in which they lie.
将成人的关节软骨在培养条件下维持三天，并在最初的48小时内用35SO4进行标记。将外植体在低温恒温器上水平切片，并分别分析顶层、中层和深层的切片。每隔二十个切片制备放射自显影片。三层中每一层的DNA含量处于同一水平，但表面的硫酸盐摄取量和糖醛酸含量显著降低。这表明软骨细胞合成与其所在层相适应的基质。
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