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经吐温80处理后对核苷酸具有通透性的中国仓鼠卵巢细胞中的DNA复制。

DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment.

作者信息

Billen D, Olson A C

出版信息

J Cell Biol. 1976 Jun;69(3):732-6. doi: 10.1083/jcb.69.3.732.

DOI:10.1083/jcb.69.3.732
PMID:1270517
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2109700/
Abstract

We have developed a method for permeabilizing CHO cells to nucleotides under conditions which allow most cells to remain viable. Permeabilized cells can carry out ATP-dependent, semiconservative synthesis of DNA. The data are consistent with the continuation of DNA synthesis in those cells in S phase at the time of treatment, possibly limited to completion of replicon synthesis without new initiations.

摘要

我们已经开发出一种方法，可在能使大多数细胞保持存活的条件下使中国仓鼠卵巢（CHO）细胞对核苷酸通透。通透后的细胞能够进行依赖ATP的DNA半保留合成。这些数据与处理时处于S期的那些细胞中DNA合成的持续情况相符，可能仅限于复制子合成的完成而无新的起始。

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DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment.经吐温80处理后对核苷酸具有通透性的中国仓鼠卵巢细胞中的DNA复制。
J Cell Biol. 1976 Jun;69(3):732-6. doi: 10.1083/jcb.69.3.732.
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[The effect of Tween 80 on the osmotic resistance of human erythrocytes].
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Animal cells reversibly permeable to small molecules.动物细胞对小分子具有可逆渗透性。
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Biochim Biophys Acta. 1976 Sep 20;447(1):65-75. doi: 10.1016/0005-2787(76)90096-4.
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引用本文的文献

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Organellar DNA synthesis in permeabilized soybean cells.线粒体 DNA 在通透化的大豆细胞中的合成。
Plant Mol Biol. 1986 Sep;7(5):331-41. doi: 10.1007/BF00032563.
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Permeable embryonic cells of sea urchins as a model for studying nucleus-cytoplasm interactions.
Experientia. 1983 Feb 15;39(2):189-90. doi: 10.1007/BF01958893.

本文引用的文献

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