经吐温80处理后对核苷酸具有通透性的中国仓鼠卵巢细胞中的DNA复制。
DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment.
作者信息
Billen D, Olson A C
出版信息
J Cell Biol. 1976 Jun;69(3):732-6. doi: 10.1083/jcb.69.3.732.
Abstract
We have developed a method for permeabilizing CHO cells to nucleotides under conditions which allow most cells to remain viable. Permeabilized cells can carry out ATP-dependent, semiconservative synthesis of DNA. The data are consistent with the continuation of DNA synthesis in those cells in S phase at the time of treatment, possibly limited to completion of replicon synthesis without new initiations.
摘要
我们已经开发出一种方法，可在能使大多数细胞保持存活的条件下使中国仓鼠卵巢（CHO）细胞对核苷酸通透。通透后的细胞能够进行依赖ATP的DNA半保留合成。这些数据与处理时处于S期的那些细胞中DNA合成的持续情况相符，可能仅限于复制子合成的完成而无新的起始。
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本文引用的文献
1
THE EFFECTS OF TWEEN 80 ON THE IN VITRO METABOLISM OF CELLS OF THE EHRLICH-LETTR'E ASCITES CARCINOMA.吐温80对艾氏腹水癌细胞体外代谢的影响
Cancer Res. 1965 Jun;25:764-9.
2
Thymine deficiency and the normal DNA replication cycle. I.胸腺嘧啶缺乏与正常DNA复制周期。I.
J Mol Biol. 1961 Apr;3:144-55. doi: 10.1016/s0022-2836(61)80041-7.
3
On the mechanism of DNA replication in mammalian chromosomes.关于哺乳动物染色体中DNA复制的机制
J Mol Biol. 1968 Mar 14;32(2):327-41. doi: 10.1016/0022-2836(68)90013-2.
4
X-ray-induced perturbations in the replication of the bacterial chromosome.X射线诱导的细菌染色体复制中的扰动。
Biochim Biophys Acta. 1965 Jul 15;103(3):440-54. doi: 10.1016/0005-2787(65)90137-1.
5
Effects of toluene on Escherichia coli.甲苯对大肠杆菌的影响。
J Bacteriol. 1965 Nov;90(5):1420-5. doi: 10.1128/jb.90.5.1420-1425.1965.
6
Programmed synthesis of deoxyribonucleic acid during the cell cycle.细胞周期中脱氧核糖核酸的程序性合成。
In Vitro. 1971 Jan-Feb;6(4):309-21. doi: 10.1007/BF02625945.
7
Replication and repair of DNA in cells of Escherichia coli treated with toluene.用甲苯处理的大肠杆菌细胞中DNA的复制与修复
Proc Natl Acad Sci U S A. 1970 Oct;67(2):674-81. doi: 10.1073/pnas.67.2.674.
8
Modification of replicon operation in HeLa cells by 2,4-dinitrophenol.2,4-二硝基苯酚对HeLa细胞中复制子操作的修饰作用。
J Mol Biol. 1973 Nov 5;80(3):393-403. doi: 10.1016/0022-2836(73)90411-7.
9
DNA replication: direction and rate of chain growth in mammalian cells.DNA复制：哺乳动物细胞中链生长的方向和速率
J Cell Biol. 1973 Aug;58(2):410-8. doi: 10.1083/jcb.58.2.410.
10
A permeable cell system for studying DNA replication in synchronized HeLa cells.
Biochim Biophys Acta. 1975 Feb 10;378(3):333-43. doi: 10.1016/0005-2787(75)90178-1.
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