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Comparison between inactivated Sendai virus, polyethylene glycol and Tween 80 as permeabilizing agents for introduction of neurospora endonuclease into X-irradiated cells.

作者信息

Obe G, Natarajan A T

出版信息

Mutat Res. 1980 Jun;71(1):133-8. doi: 10.1016/0027-5107(80)90013-5.

DOI:10.1016/0027-5107(80)90013-5
PMID:6248776
Abstract

Inactivated Sendai virus, polyethylene glycol and Tween 80 were employed as agents to make X-irradiated CHO cells permeable for Neurospora endonuclease, in studies designed to evaluate the influence of this enzyme on the frequencies of X-ray-induced chromosome aberrations. Polyethylene glycol and Tween 80 were found not to be very efficient in making cells permeable. Besides, polyethylene glycol was found to increase the frequencies of X-ray-induced chromosomal aberrations.

摘要

相似文献

1
Comparison between inactivated Sendai virus, polyethylene glycol and Tween 80 as permeabilizing agents for introduction of neurospora endonuclease into X-irradiated cells.
Mutat Res. 1980 Jun;71(1):133-8. doi: 10.1016/0027-5107(80)90013-5.
2
Molecular mechanisms involved in the production of chromosomal aberrations. II. Utilization of Neurospora endonuclease for the study of aberration production by X-rays in G1 and G2 stages of the cell cycle.染色体畸变产生所涉及的分子机制。II. 利用粗糙脉孢菌内切酶研究细胞周期G1期和G2期X射线诱导的畸变产生情况。
Mutat Res. 1980 Feb;69(2):293-305. doi: 10.1016/0027-5107(80)90094-9.
3
Molecular mechanisms involved in the production of chromosomal aberrations. I. Utilization of Neurospora endonuclease for the study of aberration production in G2 stage of the cell cycle.
Mutat Res. 1978 Oct;52(1):137-49. doi: 10.1016/0027-5107(78)90102-1.
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DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment.经吐温80处理后对核苷酸具有通透性的中国仓鼠卵巢细胞中的DNA复制。
J Cell Biol. 1976 Jun;69(3):732-6. doi: 10.1083/jcb.69.3.732.
5
On the origin of chromosomal aberrations in human peripheral lymphocytes in vitro. I. Experiments with Neurospora endonuclease and polyethylene glycol.体外培养的人外周血淋巴细胞染色体畸变的起源。I. 用粗糙脉孢菌核酸内切酶和聚乙二醇进行的实验。
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6
G1 Premature Chromosome Condensation (PCC) Assay.G1期染色体早熟凝集(PCC)检测
Methods Mol Biol. 2019;1984:31-38. doi: 10.1007/978-1-4939-9432-8_4.
7
Interaction between ceruloplasmin and Sendai virus envelope components. Note ii. Some characteristics of Tween 20-solubilized envelope components isolated by gel-filtration.铜蓝蛋白与仙台病毒包膜成分之间的相互作用。注释二。通过凝胶过滤分离的吐温20增溶包膜成分的一些特性。
Virologie. 1976 Apr-Jun;27(2):127-32.
8
Use of the 5-bromodeoxyuridine-labelling technique for exploring mechanisms involved in the formation of chromosomal aberrations.使用5-溴脱氧尿苷标记技术探索染色体畸变形成所涉及的机制。
Mutat Res. 1980 Dec;73(2):307-17. doi: 10.1016/0027-5107(80)90196-7.
9
Technical note: comparison of yields and repair kinetics of interphase chromosome breaks visualized by Sendai-virus or PEG-mediated cell fusion in irradiated CHO cells.技术说明:在经辐射的中国仓鼠卵巢细胞中,通过仙台病毒或聚乙二醇介导的细胞融合观察到的间期染色体断裂的产量和修复动力学比较。
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10
The use of Tween-80-permeabilized mammalian cells in studies of nucleic acid metabolism.
Methods Cell Biol. 1978;20:315-24.

引用本文的文献

1
Has diagnostic ultrasound mutagenic effects?诊断性超声有诱变作用吗?
Hum Genet. 1980;56(1):95-8. doi: 10.1007/BF00281576.
2
On the origin of chromosomal aberrations in human peripheral lymphocytes in vitro. I. Experiments with Neurospora endonuclease and polyethylene glycol.体外培养的人外周血淋巴细胞染色体畸变的起源。I. 用粗糙脉孢菌核酸内切酶和聚乙二醇进行的实验。
Hum Genet. 1984;66(4):335-43. doi: 10.1007/BF00287637.