Bartholomew J C, Yokota H, Ross P
J Cell Physiol. 1976 Jul;88(3):277-86. doi: 10.1002/jcp.1040880303.
The effect of serum on the growth properties of non-transformed Balb 3T3 A31 and SV40-transformed Balb 3T3 A31 was studied. The concentration of serum in the growth medium of non-transformed cells had little effect on the initial population doubling time, but did regulate the cell density at which the population became quiescent in G1. The doubling time of transformed cells, however, was increased significantly as the concentration of serum was decreased below 4%. This effect on the growth of transformed cells was seen at serum concentrations so low that non-transformed cells did not complete one population doubling. Flow microfluorometric analysis of these populations indicated that the primary effect of different serum concentrations on the non-transformed cells was to modulate the average residence time in G1, whereas, all the cell cycle phases of the transformed cells were affected by serum. At saturation densities, the non-transformed cells became quiescent in G1, but the transformed cells still traversed the cell cycle and their saturation density appeared to be a balance between cell production and cell death occurring primarily in the G1 phase of the cell cycle.
研究了血清对未转化的Balb 3T3 A31细胞和SV40转化的Balb 3T3 A31细胞生长特性的影响。未转化细胞生长培养基中血清的浓度对初始群体倍增时间影响很小，但确实调节了群体在G1期进入静止状态时的细胞密度。然而，当血清浓度降至4%以下时，转化细胞的倍增时间显著增加。在血清浓度如此之低以至于未转化细胞无法完成一次群体倍增的情况下，仍可观察到对转化细胞生长的这种影响。对这些细胞群体进行的流式细胞荧光分析表明，不同血清浓度对未转化细胞的主要作用是调节其在G1期的平均停留时间，而血清会影响转化细胞的所有细胞周期阶段。在饱和密度下，未转化细胞在G1期进入静止状态，但转化细胞仍能穿越细胞周期，其饱和密度似乎是细胞产生与主要发生在细胞周期G1期的细胞死亡之间的平衡。
