血清刺激3T3细胞中磷脂酶A2和前列腺素释放与血小板衍生的生长促进活性相关。

Serum stimulation of phospholipase A2 and prostaglandin release in 3T3 cells is associated with platelet-derived growth-promoting activity.

作者信息

Shier W T

出版信息

Proc Natl Acad Sci U S A. 1980 Jan;77(1):137-41. doi: 10.1073/pnas.77.1.137.

DOI:10.1073/pnas.77.1.137

PMID:6928609

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC348223/

Abstract

Sera from mouse, rat, and calf sources stimulate cellular phospholipase A2 activity (PLase; phosphatide 2-acylhydrolase, EC 3.1.1.4) and prostaglandin synthesis in 3T3 Swiss mouse fibroblasts, releasing up to 33% of biosynthetically incorporated [3H]arachidonic acid as hydrolysis products in 1 hr. The PLase stimulated by mouse serum exhibits specificity for arachidonic acid residues on phospholipids. It is stimulated 2.5-fold by 1.8 mM Ca2+ in the presence of 5 microM divalent cation ionophore A23187, consistent with a Ca2+-dependent enzyme possessing a cytoplasmic Ca2+ binding site. The percentage maximal PLase- and growth-stimulating activities of the three sera exhibit similar concentration dependencies, with the homologous (mouse) serum exhibiting the highest specific activities. Confluent 3T3 cells deplete PLase-stimulating activity from medium containing calf serum at a rate similar to the depletion of cell growth-stimulus activity. The PLase-stimulating activity in rat and mouse sera is derived from the leukocyte fraction of blood, presumably from platelets. In rat leukocyte lysates the PLase-stimulating activity exhibits properties similar to those reported for platelet-derived cell growth factors from rat and human sources--i.e., stability to exposure to 100 degrees C for 2 min or to pH 2 or pH 11, and cationic properties. Purified preparations of human platelet-derived growth factor also exhibit 3T3 PLase-stimulating activity.

摘要

来自小鼠、大鼠和小牛的血清可刺激3T3瑞士小鼠成纤维细胞中的细胞磷脂酶A2活性（PLase；磷脂2-酰基水解酶，EC 3.1.1.4）和前列腺素合成，在1小时内可释放高达33%的生物合成掺入的[3H]花生四烯酸作为水解产物。小鼠血清刺激的PLase对磷脂上的花生四烯酸残基具有特异性。在5 microM二价阳离子载体A23187存在下，1.8 mM Ca2+可使其活性提高2.5倍，这与一种具有细胞质Ca2+结合位点的Ca2+依赖性酶一致。三种血清的最大PLase刺激活性和生长刺激活性百分比表现出相似的浓度依赖性，同源（小鼠）血清表现出最高的比活性。汇合的3T3细胞以类似于细胞生长刺激活性消耗的速率从含有小牛血清的培养基中消耗PLase刺激活性。大鼠和小鼠血清中的PLase刺激活性源自血液中的白细胞部分，可能来自血小板。在大鼠白细胞裂解物中，PLase刺激活性表现出与报道的来自大鼠和人类来源的血小板衍生细胞生长因子相似的特性——即对暴露于100℃2分钟或pH 2或pH 11稳定，以及具有阳离子特性。纯化的人血小板衍生生长因子制剂也表现出3T3 PLase刺激活性。

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