Ackermann Paul W, Li Jian, Lundeberg Thomas, Kreicbergs Andris
Department of Surgical Sciences, Orthopedic Laboratory, Research Center M3:02, Karolinska Hospital, S-171 76 Stockholm, Sweden.
J Orthop Res. 2003 May;21(3):432-41. doi: 10.1016/S0736-0266(02)00207-3.
Nerve regeneration and the occurrence of three neuropeptides; i.e. substance P (SP), calcitonin gene related peptide (CGRP) and galanin (GAL), were studied during healing of tendon rupture in the rat by semi-quantitative immunohistochemistry. The neuronal findings were related to nociception as assessed by hindpaw withdrawal latencies at thermal and mechanical tests. Experimental rupture of rat Achilles tendon--normally devoid of nerves--elicited extensive nerve ingrowth into the rupture site in the early phase of healing followed by almost complete fiber disappearance (weeks 12-16). The ingrowth of SP and CGRP positive fibers, seen already at weeks 1-2, was associated with increased nociception. Subsequently, the occurrence of GAL positive fibers at weeks 4-6 was associated with decreased nociception. An even stronger relationship to nociception during healing was observed when the rate of change in neuropeptide expression instead of the expression in absolute terms was considered, according to the "cascade" formula of SP(')+CGRP(')-GAL(').It may prove that the observed temporal occurrence of different neuropeptides reflects a role of the peripheral nervous system in regulating synchronously nociception and healing.
通过半定量免疫组织化学方法，研究了大鼠跟腱断裂愈合过程中的神经再生以及三种神经肽（即P物质（SP）、降钙素基因相关肽（CGRP）和甘丙肽（GAL））的出现情况。通过热刺激和机械刺激测试后爪退缩潜伏期来评估神经元的研究结果与伤害感受相关。大鼠跟腱实验性断裂（正常情况下没有神经）在愈合早期引发大量神经长入断裂部位，随后几乎完全消失（12 - 16周）。在第1 - 2周就可见到的SP和CGRP阳性纤维的长入与伤害感受增加有关。随后，在第4 - 6周出现的GAL阳性纤维与伤害感受降低有关。根据SP(')+CGRP(')-GAL(')的“级联”公式，当考虑神经肽表达的变化率而非绝对表达量时，观察到愈合过程中与伤害感受有更强的相关性。可能证明观察到的不同神经肽的时间性出现反映了外周神经系统在同步调节伤害感受和愈合中的作用。
