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跟腱完全断裂可诱导小鼠脊髓产生炎症反应和神经胶质细胞活化。

Total rupture of Achilles tendon induces inflammatory response and glial activation on the spinal cord of mice.

机构信息

Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.

Instituto de Ciências da Saúde, Universidade Federal do Pará, Belém, PA, Brasil.

出版信息

Braz J Med Biol Res. 2023 Oct 13;56:e12391. doi: 10.1590/1414-431X2023e12391. eCollection 2023.

DOI:10.1590/1414-431X2023e12391
PMID:37851789
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10578131/
Abstract

Rupture of Achilles tendon is a common accident affecting professional and recreational athletes. Acute and chronic pain are symptoms commonly observed in patients with rupture. However, few studies have investigated whether Achilles tendon rupture is able to promote disorders in the central nervous system (CNS). Therefore, the current study aimed to evaluate nociceptive alterations and inflammatory response in the L5 lumbar segment of Balb/c mice spinal cord after Achilles tendon rupture. We found increased algesia in the paw of the ruptured group on the 7th and 14th days post-tenotomy compared with the control group. This phenomenon was accompanied by overexpression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase-2 (NOS-2) as well as hyperactivation of astrocytes and microglia in nociceptive areas of L5 spinal cord as evidenced by intense GFAP and IBA-1 immunostaining, respectively. Biochemical studies also demonstrated increased levels of nitrite in the L5 spinal cord of tenotomized animals compared with the control group. Thus, we have demonstrated for the first time that total rupture of the Achilles tendon induced inflammatory response and nitrergic and glial activation in the CNS in the L5 spinal cord region.

摘要

跟腱断裂是一种常见的意外伤害，影响专业和业余运动员。急性和慢性疼痛是跟腱断裂患者常见的症状。然而，很少有研究调查跟腱断裂是否能够促进中枢神经系统（CNS）的紊乱。因此，本研究旨在评估跟腱断裂后 Balb/c 小鼠 L5 腰椎段脊髓中的伤害感受改变和炎症反应。我们发现，与对照组相比，跟腱切断后第 7 天和第 14 天，实验组小鼠受损侧足部出现痛觉过敏。这种现象伴随着环氧合酶-2（COX-2）和诱导型一氧化氮合酶-2（NOS-2）的过度表达，以及伤害感受区 L5 脊髓中的星形胶质细胞和小胶质细胞的过度激活，分别通过强烈的 GFAP 和 IBA-1 免疫染色证实。生化研究还表明，与对照组相比，实验组动物 L5 脊髓中的亚硝酸盐水平升高。因此，我们首次证明，跟腱完全断裂会导致中枢神经系统 L5 脊髓区域的炎症反应和硝化及神经胶质激活。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/77add487aa45/1414-431X-bjmbr-56-e12391-gf010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/72dc72058229/1414-431X-bjmbr-56-e12391-gf001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/7dfb9fe3b502/1414-431X-bjmbr-56-e12391-gf003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/51da7113357f/1414-431X-bjmbr-56-e12391-gf004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/b819056535c7/1414-431X-bjmbr-56-e12391-gf005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/5ec6d9ceb02e/1414-431X-bjmbr-56-e12391-gf006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/a16f5f735356/1414-431X-bjmbr-56-e12391-gf007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/8b4295a513bf/1414-431X-bjmbr-56-e12391-gf008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/5cbd4c9856fd/1414-431X-bjmbr-56-e12391-gf009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/77add487aa45/1414-431X-bjmbr-56-e12391-gf010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/72dc72058229/1414-431X-bjmbr-56-e12391-gf001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/d2e904380b30/1414-431X-bjmbr-56-e12391-gf002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/7dfb9fe3b502/1414-431X-bjmbr-56-e12391-gf003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/51da7113357f/1414-431X-bjmbr-56-e12391-gf004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/b819056535c7/1414-431X-bjmbr-56-e12391-gf005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/5ec6d9ceb02e/1414-431X-bjmbr-56-e12391-gf006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/a16f5f735356/1414-431X-bjmbr-56-e12391-gf007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/8b4295a513bf/1414-431X-bjmbr-56-e12391-gf008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/5cbd4c9856fd/1414-431X-bjmbr-56-e12391-gf009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e483/10578131/77add487aa45/1414-431X-bjmbr-56-e12391-gf010.jpg

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