Müller-Reichert Thomas, Sassoon Ingrid, O'Toole Eileen, Romao Maryse, Ashford Anthony J, Hyman Anthony A, Antony Claude
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
Chromosoma. 2003 Apr;111(7):417-28. doi: 10.1007/s00412-002-0220-6. Epub 2003 Mar 18.
Ndc10p is one of the DNA-binding constituents of the kinetochore in Saccharomyces cerevisiae but light microscopy analysis suggests that Ndc10p is not limited to kinetochore regions. We examined the localization of Ndc10p using immunoelectron microscopy and showed that Ndc10p is associated with spindle microtubules from S-phase through anaphase. By serial section reconstruction of mitotic spindles combined with immunogold detection, we showed that Ndc10p interacts with microtubules laterally as well as terminally. About 50% of the gold label in serial section reconstructions of short mitotic spindles was associated with the walls of spindle microtubules. Interaction of kinetochore components with microtubule walls was also shown for kinetochore protein Ndc80p. Our data suggest that at least a subset of kinetochore-associated protein is dispersed throughout the mitotic spindle.
Ndc10p是酿酒酵母中动粒的DNA结合成分之一，但光学显微镜分析表明，Ndc10p并不局限于动粒区域。我们使用免疫电子显微镜检查了Ndc10p的定位，结果显示从S期到后期，Ndc10p都与纺锤体微管相关联。通过对有丝分裂纺锤体进行连续切片重建并结合免疫金检测，我们发现Ndc10p不仅在微管末端，也在微管侧面与微管相互作用。在短有丝分裂纺锤体的连续切片重建中，约50%的金标记与纺锤体微管的管壁相关联。动粒蛋白Ndc80p也显示出其动粒成分与微管壁相互作用。我们的数据表明，至少一部分与动粒相关的蛋白质分散在整个有丝分裂纺锤体中。
