Jaeger H, Klör H U, Ditschuneit H
J Lipid Res. 1976 Mar;17(2):185-90.
The availability of an excellent separation method for fatty acid methyl esters, including separation of cis and trans isomers and of isomers that differ only in the position of double bonds, has become more and more important. The present glass capillary chromatography system combines high separation power with high precision and easy handling. Moreover, the system is completely automated and therefore provides a time saving method. As compared to a conventional packed column, the glass capillary column provides about one hundred fold more theoretical plates (227,000), as well as narrower peaks, thus giving rise to less error when integrating with electronic integrators. The reproducibility for relative retention time is better with the capillary column (0.26%) and reproducibility of the weight percent values is at least similar to that of the packed column (1.53%). When handling only small sample amounts the capillary provides better values because of its low capacity. This powerful system should open up new possibilities in the field of fatty acid investigation.
对于脂肪酸甲酯而言，拥有一种出色的分离方法变得越来越重要，这种方法要能够分离顺式和反式异构体以及仅在双键位置上不同的异构体。目前的玻璃毛细管色谱系统将高分离能力与高精度及易于操作相结合。此外，该系统完全自动化，因此提供了一种节省时间的方法。与传统填充柱相比，玻璃毛细管柱提供的理论塔板数大约多一百倍（227,000），并且峰更窄，因此在与电子积分仪积分时产生的误差更小。毛细管柱相对保留时间的重现性更好（0.26%），重量百分比值的重现性至少与填充柱相似（1.53%）。当仅处理少量样品时，由于毛细管柱容量低，其表现出更好的值。这种强大的系统应该会在脂肪酸研究领域开辟新的可能性。
