Haug M, Dieterich I, Laubach C, Reinhardt D, Harzer G
J Chromatogr. 1983 Nov 25;279:549-53. doi: 10.1016/s0021-9673(01)93656-8.
The fatty acid (FA) composition of the human milk lipid subclasses sphingomyelin, phosphatidylcholine, phosphatidylethanolamine and cholesterol esters (CE) were analysed by capillary gas chromatography (GC) on wall-coated open-tubular glass columns. Compared with GC on packed columns, capillary GC was found to be ten times more sensitive (0.1 microgram of each individual FA methyl ester could be quantified), and the time needed for the analysis could be reduced by a factor of five. The reproducibility of the analysis was good relative standard deviation (4-7%) and comparable to that obtained by packed column GC.
采用壁涂开管玻璃柱上的毛细管气相色谱法（GC）分析了人乳脂质亚类鞘磷脂、磷脂酰胆碱、磷脂酰乙醇胺和胆固醇酯（CE）的脂肪酸（FA）组成。与填充柱GC相比，毛细管GC的灵敏度提高了10倍（每种FA甲酯的定量限为0.1微克），分析所需时间可缩短5倍。该分析的重现性良好，相对标准偏差为4%-7%，与填充柱GC获得的结果相当。
