Shownkeen R C, Hartree A S, Stewart F, Mashiter K, Stevens V C
J Endocrinol. 1976 May;69(2):263-73. doi: 10.1677/joe.0.0690263.
Highly purified human pituitary FSH was partially dissociated by treatment with 8M-urea, and alpha- and beta-subunits were isolated by ion-exchange chromatography and gel filtration. Tests of biological activity by in-vivo assays and in-vitro radioreceptor assays were in good agreement and showed that preparations of isolated alpha-subunit had less than 1%, and beta-subunit from 2 to 10% of the FSH activity of the intact hormone. In contrast to results reported elsewhere, most of the subunit preparations reassociated with counterpart subunit to regain biological activity equal to that of intact FSH (around 160 mg NIH-FSH-S1/mg). The intact FSH recovered as a by-product after isolation of subunits was of high biological activity, and its LH contamination was reduced by more than 90% when compared with thepurified FSH starting material. The subunits are relatively inactive in a radioimmunoassay specific for intact FSH. Sialic acid and tryptophan determinations indicated that both subunits contain sialic acid and that tryptophan is present only in the beta-subunit.
用8M尿素处理高度纯化的人垂体促卵泡激素（FSH）使其部分解离，通过离子交换色谱法和凝胶过滤法分离出α亚基和β亚基。体内试验和体外放射受体试验对生物活性的检测结果高度一致，结果表明，分离得到的α亚基制剂的活性低于完整激素FSH活性的1%，β亚基的活性为完整激素FSH活性的2%至10%。与其他地方报道的结果相反，大多数亚基制剂与相应亚基重新结合，恢复的生物活性与完整FSH相当（约160mg NIH-FSH-S1/mg）。亚基分离后作为副产物回收的完整FSH具有高生物活性，与纯化的起始FSH原料相比，其促黄体生成素（LH）污染减少了90%以上。在针对完整FSH的放射免疫分析中，亚基相对无活性。唾液酸和色氨酸测定表明，两个亚基均含有唾液酸，且色氨酸仅存在于β亚基中。
