通过逆转录聚合酶链反应和荧光共振能量转移探针检测猫杯状病毒分离株中的核苷酸多态性

Detection of nucleotide polymorphisms in feline calicivirus isolates by reverse transcription PCR and a fluorescence resonance energy transfer probe.

作者信息

Helps Chris, Harbour Dave

机构信息

Division of Veterinary Pathology, Infection and Immunity, Department of Clinical Veterinary Science, University of Bristol, Langford House, Langford, Bristol BS40 5DU, UK.

出版信息

J Virol Methods. 2003 May;109(2):261-3. doi: 10.1016/s0166-0934(03)00051-x.

DOI:10.1016/s0166-0934(03)00051-x

PMID:12711071

Abstract

A fluorescence resonance energy transfer system has been developed to detect nucleotide polymorphisms in isolates of feline calicivirus (FCV). Isolates with an exact match to the 28 nucleotide probe gave a melting temperature of 75 degrees C while isolates with one, two, three or four mismatches gave melting temperatures of 71, 65, 60 and 57 degrees C, respectively. The technique, which is simple, rapid and accurate, is suitable for typing field isolates of FCV.

摘要

已开发出一种荧光共振能量转移系统，用于检测猫杯状病毒（FCV）分离株中的核苷酸多态性。与28个核苷酸探针完全匹配的分离株的解链温度为75摄氏度，而有一个、两个、三个或四个错配的分离株的解链温度分别为71、65、60和57摄氏度。该技术简单、快速且准确，适用于FCV野外分离株的分型。