Nayak Sunil V, Shivarudrappa A S, Mukkamil Ahmed Siddiq
Department of Pathology, Victoria Hospital, Fort, Bangalore, India.
Ann Diagn Pathol. 2003 Apr;7(2):78-81. doi: 10.1053/adpa.2003.50012.
We compared the sensitivity of the fluorescent method with that of he modified Fite-Faraco method in the detection of Mycobacterium leprae in tissue sections. Fifty-six skin biopsies were obtained from patients having leprosy, particularly the paucibacillary type. Minor alterations were made in the deparaffinization and staining technique, as compared with Kuper and May's method, to obtain optimum fluorescence. Of 56 biopsies studied, 39 showed organisms by the fluorescent method and only 25 showed organisms by the modified Fite-Faraco method. The fluorescent method was found to be more advantageous than the modified Fite-Faraco method, particularly in paucibacillary cases. Fluorescent microscopy has the advantage of speed and ease of screening and reduces observer fatigue. Bacillary positivity rates were higher in the fluorescent method than in the modified Fite-Faraco method in each type of leprosy.
我们比较了荧光法与改良菲-法罗科法在检测组织切片中麻风分枝杆菌时的敏感性。从麻风患者，尤其是少菌型患者身上获取了56份皮肤活检样本。与库珀和梅的方法相比，在脱石蜡和染色技术上做了细微改动，以获得最佳荧光效果。在所研究的56份活检样本中，荧光法检测出有菌的样本为39份，而改良菲-法罗科法仅检测出25份。结果发现荧光法比改良菲-法罗科法更具优势，尤其是在少菌型病例中。荧光显微镜检查具有速度快、易于筛查的优点，还能减少观察者的疲劳。在各型麻风病中，荧光法的杆菌阳性率均高于改良菲-法罗科法。
