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用于检测组织切片中麻风杆菌的荧光显微镜检查。

Fluorescence microscopy for detection of M. leprae in tissue sections.

作者信息

Jariwala H J, Kelkar S S

出版信息

Int J Lepr Other Mycobact Dis. 1979 Mar;47(1):33-6.

PMID:376453
Abstract

The fluorescence method was compared with the Fite-Faraco method for detecting acid-fast microorganisms in paraffin sections of cases of leprosy. Biopsies were obtained from 50 cases of leprosy covering all varieties and at varying stages of treatment. The fluorescence method was better than the Fite-Faraco method; 22 biopsies showing acid-fast organisms in fluorescence microscopy and 20 in the Fite-Faraco method. Its superiority was evidenced in two cases in which the organisms were very scanty. Fluorescence microscopy can also be used to determine the Bacterial Index and the Morphologic Index of organisms. The Morphologic Index, however, was one and a half times higher than that obtained by the Fite-Faraco technic. The ease and speed of fluorescence microscopy appear to be a great advantage.

摘要

将荧光法与菲-法氏(Fite-Faraco)法用于检测麻风病例石蜡切片中的抗酸微生物进行了比较。从50例涵盖所有类型且处于不同治疗阶段的麻风病例中获取活检组织。荧光法优于菲-法氏法;荧光显微镜检查显示有抗酸菌的活检组织为22例,而菲-法氏法为20例。在两例抗酸菌极少的病例中证明了其优越性。荧光显微镜检查还可用于确定细菌指数和微生物的形态学指数。然而,形态学指数比用菲-法氏技术获得的指数高1.5倍。荧光显微镜检查的简便性和速度似乎是一大优势。

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Fluorescence microscopy for detection of M. leprae in tissue sections.用于检测组织切片中麻风杆菌的荧光显微镜检查。
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引用本文的文献

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Fluorescent Microscopy: A Useful and Adjunct Tool in Leprosy Diagnosis: A Cross Sectional Study.荧光显微镜检查:麻风病诊断中的一种有用辅助工具:一项横断面研究
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Evaluation of Fluorescent Staining for Diagnosis of Leprosy and its Impact on Grading of the Disease: Comparison with Conventional Staining.荧光染色用于麻风病诊断及其对疾病分级影响的评估:与传统染色的比较
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