Cole C H, Sella G
Pediatr Res. 1975 Oct;9(10):763-6. doi: 10.1203/00006450-197510000-00003.
A study has been made of the effect of saliva from children with cystic fibrosis of the pancreas (CFP) on various components of the ATP hydrolyzing enzyme system. The ouabain-sensitive ATPase activity of erythrocyte membranes prepared from intact erythrocytes preincubated with CFP saliva was 35 +/- 4 nmol Pi/mg fry wt membrane suspension/hr, compared with 48 +/- 7 nmol Pi/mg dry wt membrane suspension/hr when the erythrocytes were preincubated with control saliva. A calcium-activated component of ATPase was decreased from 202 +/- 30 nmol Pi/mg dry wt membrane suspension/hr in erythrocytes preincubated with control saliva, to 151 +/- 17 nmol Pi/mg dry wt membrane suspension/hr when the incubation was carried out with CFP saliva. In a second series of experiments, ultrafiltered saliva was added directly to an ATPase assay. The saliva from children with CFP brought about a mean decrease in ouabain-sensitive ATPase of 16% compared with control saliva.
对患有胰腺囊性纤维化（CFP）儿童的唾液对ATP水解酶系统各组分的影响进行了一项研究。用CFP唾液预孵育完整红细胞制备的红细胞膜的哇巴因敏感ATP酶活性为35±4 nmol Pi/mg干重膜悬浮液/小时，而用对照唾液预孵育红细胞时为48±7 nmol Pi/mg干重膜悬浮液/小时。ATP酶的钙激活组分从用对照唾液预孵育的红细胞中的202±30 nmol Pi/mg干重膜悬浮液/小时，降至用CFP唾液孵育时的151±17 nmol Pi/mg干重膜悬浮液/小时。在第二系列实验中，将超滤唾液直接加入ATP酶测定中。与对照唾液相比，CFP儿童的唾液使哇巴因敏感ATP酶平均降低了16%。
