健康成年人中疱疹病毒和多瘤病毒再激活及脱落的动态变化：一项为期14个月的纵向研究。

The dynamics of herpesvirus and polyomavirus reactivation and shedding in healthy adults: a 14-month longitudinal study.

作者信息

Ling Paul D, Lednicky John A, Keitel Wendy A, Poston David G, White Zoe S, Peng RongSheng, Liu Zhensheng, Mehta Satish K, Pierson Duane L, Rooney Cliona M, Vilchez Regis A, Smith E O'Brian, Butel Janet S

机构信息

Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030, USA.

出版信息

J Infect Dis. 2003 May 15;187(10):1571-80. doi: 10.1086/374739. Epub 2003 Apr 30.

DOI:10.1086/374739

PMID:12721937

Abstract

Humans are infected with viruses that establish long-term persistent infections. To address whether immunocompetent individuals control virus reactivation globally or independently and to identify patterns of sporadic reactivation, we monitored herpesviruses and polyomaviruses in 30 adults, over 14 months. Epstein-Barr virus (EBV) DNA was quantitated in saliva and peripheral blood mononuclear cells (PBMCs), cytomegalovirus (CMV) was assayed in urine, and JC virus (JCV) and BK virus (BKV) DNAs were assayed in urine and PBMCs. All individuals shed EBV in saliva, whereas 67% had >or=1 blood sample positive for EBV. Levels of EBV varied widely. CMV shedding occurred infrequently but occurred more commonly in younger individuals (P<.03). JCV and BKV virurias were 46.7% and 0%, respectively. JCV shedding was age dependent and occurred commonly in individuals >or=40 years old (P<.03). Seasonal variation was observed in shedding of EBV and JCV, but there was no correlation among shedding of EBV, CMV, and JCV (P>.50). Thus, adults independently control persistent viruses, which display discordant, sporadic reactivations.

摘要

人类会感染能建立长期持续性感染的病毒。为了探究免疫功能正常的个体是在整体上还是独立地控制病毒再激活，并确定散发性再激活的模式，我们在14个多月的时间里对30名成年人的疱疹病毒和多瘤病毒进行了监测。对唾液和外周血单核细胞（PBMC）中的爱泼斯坦 - 巴尔病毒（EBV）DNA进行定量分析，对尿液中的巨细胞病毒（CMV）进行检测，对尿液和PBMC中的JC病毒（JCV）和BK病毒（BKV）DNA进行检测。所有个体唾液中均有EBV排出，而67%的个体有≥1份血液样本EBV呈阳性。EBV水平差异很大。CMV排出情况不常见，但在较年轻个体中更常见（P<0.03）。JCV和BKV病毒尿症的发生率分别为46.7%和0%。JCV排出与年龄有关，常见于≥40岁的个体（P<0.03）。观察到EBV和JCV排出存在季节性变化，但EBV、CMV和JCV的排出之间没有相关性（P>0.50）。因此，成年人独立控制持续性病毒，这些病毒表现出不一致的散发性再激活。