Hemminki K
Mol Cell Biochem. 1976 Mar 26;11(1):9-15. doi: 10.1007/BF01792830.
The effect of secondary stimulation with estrogen on synthesis of nuclear and nucleolar proteins is studied in chick oviduct. Isolated nuclei and nucleoli have a protein/DNA ratio of 5.2 and 5.6, respectively. 35% of nuclear and nucleolar protein is recovered in the histone fraction after hydroxylapatite chromatography. Gel electrophoretic separations of nuclear and nucleolar nonhistones are largely similar as to visible bands and distribution of radioactivity. Nucleoli bind 1.4 times more [3H] estradiol as compared to whole nuclei. Nucleolar histones are labelled slightly more actively with [3H] leucine than nuclear histones; nucleolar nonhistones are labelled about 3 times more actively than nuclear nonhistones. An 18 hour secondary stimulation with estrogen increases the radioactivity of histones by 6-fold and that of nonhistones by 2.5-fold in whole nuclei as well as in nucleoli. Stimulation appears to increase preferentially radioactivity of nonhistones at 50 000 daltons. As this change is observed in whole nuclei and nucleoli and is not reduced with hydroxyurea, it is suggested that this may be related to a gross structural reorganisation of chromatin induced by the hormone.
在鸡输卵管中研究了雌激素二次刺激对核蛋白和核仁蛋白合成的影响。分离出的细胞核和核仁的蛋白质/DNA比值分别为5.2和5.6。经羟基磷灰石层析后，35%的核蛋白和核仁蛋白存在于组蛋白部分。核和核仁非组蛋白的凝胶电泳分离在可见条带和放射性分布方面大致相似。与整个细胞核相比，核仁结合的[3H]雌二醇多1.4倍。核仁组蛋白被[3H]亮氨酸标记的活性略高于细胞核组蛋白；核仁非组蛋白被标记的活性约为细胞核非组蛋白的3倍。雌激素18小时的二次刺激使整个细胞核以及核仁中的组蛋白放射性增加6倍，非组蛋白放射性增加2.5倍。刺激似乎优先增加了50000道尔顿非组蛋白的放射性。由于在整个细胞核和核仁中都观察到了这种变化，并且用羟基脲处理后没有减弱，因此表明这可能与激素诱导的染色质总体结构重组有关。
